Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21021 | 3' | -49.6 | NC_004735.1 | + | 27114 | 0.67 | 0.992595 |
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Target: 5'- aCAACgCGUgCCAUaUCgUUCCUUUUUCu -3' miRNA: 3'- gGUUGgGCA-GGUGaAG-AAGGAGAAAG- -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 47755 | 0.69 | 0.97696 |
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Target: 5'- aCCAuGCuuGaUCaGCUUCUUUCUCUUUCc -3' miRNA: 3'- -GGU-UGggC-AGgUGAAGAAGGAGAAAG- -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 78511 | 0.69 | 0.971458 |
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Target: 5'- uCCAGCgCuUCUGCUUCUUCUcCUUUCc -3' miRNA: 3'- -GGUUGgGcAGGUGAAGAAGGaGAAAG- -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 69591 | 0.69 | 0.968057 |
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Target: 5'- cCCAACCCGagauuucUCCAgagccCUUCUUUCUCa--- -3' miRNA: 3'- -GGUUGGGC-------AGGU-----GAAGAAGGAGaaag -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 40039 | 0.72 | 0.885065 |
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Target: 5'- aCAACC--UCCACUcccguuUUUUCCUCUUUCa -3' miRNA: 3'- gGUUGGgcAGGUGA------AGAAGGAGAAAG- -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 84840 | 0.73 | 0.854067 |
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Target: 5'- uCCAGCCCcUCCACcaCUUCCUuCUUgcUCa -3' miRNA: 3'- -GGUUGGGcAGGUGaaGAAGGA-GAA--AG- -5' |
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| 21021 | 3' | -49.6 | NC_004735.1 | + | 92344 | 1.14 | 0.005226 |
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Target: 5'- aCCAACCCGUCCACUUCUUCCUCUUUCa -3' miRNA: 3'- -GGUUGGGCAGGUGAAGAAGGAGAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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