Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21022 | 5' | -55.7 | NC_004735.1 | + | 117840 | 0.66 | 0.903726 |
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Target: 5'- -aCCAGCgaauaGCUCAGaAUUUGCGGAGGg -3' miRNA: 3'- ggGGUUGgg---CGAGUU-UGAGCGCCUCU- -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 27598 | 0.66 | 0.903726 |
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Target: 5'- aCCCCAACggauaccauuCUGUUCAGcuACUCGCuGGAc- -3' miRNA: 3'- -GGGGUUG----------GGCGAGUU--UGAGCG-CCUcu -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 39774 | 0.67 | 0.876731 |
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Target: 5'- aCUCCAACU--CUC--GCUUGCGGAGAc -3' miRNA: 3'- -GGGGUUGGgcGAGuuUGAGCGCCUCU- -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 9501 | 0.67 | 0.861899 |
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Target: 5'- aCgCCGgaucAUCCGCaUAucCUCGCGGAGAa -3' miRNA: 3'- -GgGGU----UGGGCGaGUuuGAGCGCCUCU- -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 115160 | 0.68 | 0.829786 |
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Target: 5'- uCCCCgAACCCuuCUUuuuaUCGCGGGGAa -3' miRNA: 3'- -GGGG-UUGGGc-GAGuuugAGCGCCUCU- -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 46565 | 0.68 | 0.812616 |
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Target: 5'- aCUCCAaugaACCCGCUCAGGugguCUgGUGGAa- -3' miRNA: 3'- -GGGGU----UGGGCGAGUUU----GAgCGCCUcu -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 100607 | 0.71 | 0.63703 |
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Target: 5'- aCCCUucaaguGCCCGCUCAAACUgcauacgauaaaUGgGGAGu -3' miRNA: 3'- -GGGGu-----UGGGCGAGUUUGA------------GCgCCUCu -5' |
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| 21022 | 5' | -55.7 | NC_004735.1 | + | 92509 | 1.11 | 0.00201 |
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Target: 5'- aCCCCAACCCGCUCAAACUCGCGGAGAa -3' miRNA: 3'- -GGGGUUGGGCGAGUUUGAGCGCCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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