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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21023 | 5' | -50.2 | NC_004735.1 | + | 58548 | 0.66 | 0.98641 |
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Target: 5'- uGGCGCuuACCAGUUUacGGAAACAa--- -3' miRNA: 3'- uUCGCGu-UGGUCGAA--CCUUUGUgaag -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 99773 | 0.66 | 0.98641 |
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Target: 5'- cGGUauuuaCAACCGGCUgcuuagGGAuaGGCGCUUCa -3' miRNA: 3'- uUCGc----GUUGGUCGAa-----CCU--UUGUGAAG- -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 100320 | 0.66 | 0.98641 |
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Target: 5'- -cGCGcCAGCCGGUUgauuucGAACGCUUCu -3' miRNA: 3'- uuCGC-GUUGGUCGAacc---UUUGUGAAG- -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 58607 | 0.66 | 0.984565 |
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Target: 5'- -uGCGUAACCAgaucGCUgaagcUGGAAGcCugUUCg -3' miRNA: 3'- uuCGCGUUGGU----CGA-----ACCUUU-GugAAG- -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 29249 | 0.67 | 0.96594 |
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Target: 5'- gAAGCGCuggugcGCCAGUauUUGGAAuCACUcCg -3' miRNA: 3'- -UUCGCGu-----UGGUCG--AACCUUuGUGAaG- -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 114050 | 0.68 | 0.962355 |
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Target: 5'- aAAGCGCAACCAcCccggUGGGAACGuCUg- -3' miRNA: 3'- -UUCGCGUUGGUcGa---ACCUUUGU-GAag -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 122202 | 0.7 | 0.891254 |
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Target: 5'- cGAGCGCAAgCGGCUUG-AAACG-UUCg -3' miRNA: 3'- -UUCGCGUUgGUCGAACcUUUGUgAAG- -5' |
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| 21023 | 5' | -50.2 | NC_004735.1 | + | 105319 | 1.07 | 0.009019 |
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Target: 5'- uAAGCGCAACCAGCUUGGAAACACUUCc -3' miRNA: 3'- -UUCGCGUUGGUCGAACCUUUGUGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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