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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21025 | 3' | -53.5 | NC_004735.1 | + | 46925 | 0.66 | 0.947635 |
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Target: 5'- -aGUGAGCAGCAaugcGcCGGUGCAgcuugUAUGGGa -3' miRNA: 3'- cgCACUUGUCGU----C-GCCGUGU-----AUGCCU- -5' |
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| 21025 | 3' | -53.5 | NC_004735.1 | + | 92329 | 0.66 | 0.938295 |
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Target: 5'- aCGUGGugcucuGCAGCauucuGGCGGCugGUGuCGGc -3' miRNA: 3'- cGCACU------UGUCG-----UCGCCGugUAU-GCCu -5' |
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| 21025 | 3' | -53.5 | NC_004735.1 | + | 99916 | 0.68 | 0.88312 |
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Target: 5'- cGCGuUGAuagcucagACAGCAGCGGgugcuggagacaaCAUcgACGGAu -3' miRNA: 3'- -CGC-ACU--------UGUCGUCGCC-------------GUGuaUGCCU- -5' |
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| 21025 | 3' | -53.5 | NC_004735.1 | + | 89458 | 0.7 | 0.801478 |
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Target: 5'- aGCGUGAGuCGGCGGuuGUuuuUAUGCGGGg -3' miRNA: 3'- -CGCACUU-GUCGUCgcCGu--GUAUGCCU- -5' |
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| 21025 | 3' | -53.5 | NC_004735.1 | + | 125562 | 1.11 | 0.002887 |
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Target: 5'- gGCGUGAACAGCAGCGGCACAUACGGAg -3' miRNA: 3'- -CGCACUUGUCGUCGCCGUGUAUGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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