Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21028 | 3' | -56.7 | NC_004735.1 | + | 104586 | 0.66 | 0.874815 |
Target: 5'- aUACCGACuucauuguugaaaCAGA-CCGUCGCAuUGCGa -3' miRNA: 3'- -GUGGCUGug-----------GUCUaGGCAGCGU-GCGU- -5' |
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21028 | 3' | -56.7 | NC_004735.1 | + | 44839 | 0.68 | 0.741531 |
Target: 5'- gACCGACGgC-GAUugcaUCGUUGCACGCGg -3' miRNA: 3'- gUGGCUGUgGuCUA----GGCAGCGUGCGU- -5' |
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21028 | 3' | -56.7 | NC_004735.1 | + | 72399 | 0.69 | 0.691809 |
Target: 5'- cCGCCGACA----AUCCGUUGUAUGCAu -3' miRNA: 3'- -GUGGCUGUggucUAGGCAGCGUGCGU- -5' |
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21028 | 3' | -56.7 | NC_004735.1 | + | 126776 | 1.08 | 0.002489 |
Target: 5'- gCACCGACACCAGAUCCGUCGCACGCAg -3' miRNA: 3'- -GUGGCUGUGGUCUAGGCAGCGUGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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