miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21028 3' -56.7 NC_004735.1 + 104586 0.66 0.874815
Target:  5'- aUACCGACuucauuguugaaaCAGA-CCGUCGCAuUGCGa -3'
miRNA:   3'- -GUGGCUGug-----------GUCUaGGCAGCGU-GCGU- -5'
21028 3' -56.7 NC_004735.1 + 44839 0.68 0.741531
Target:  5'- gACCGACGgC-GAUugcaUCGUUGCACGCGg -3'
miRNA:   3'- gUGGCUGUgGuCUA----GGCAGCGUGCGU- -5'
21028 3' -56.7 NC_004735.1 + 72399 0.69 0.691809
Target:  5'- cCGCCGACA----AUCCGUUGUAUGCAu -3'
miRNA:   3'- -GUGGCUGUggucUAGGCAGCGUGCGU- -5'
21028 3' -56.7 NC_004735.1 + 126776 1.08 0.002489
Target:  5'- gCACCGACACCAGAUCCGUCGCACGCAg -3'
miRNA:   3'- -GUGGCUGUGGUCUAGGCAGCGUGCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.