Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21028 | 5' | -52.4 | NC_004735.1 | + | 28993 | 0.66 | 0.972578 |
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Target: 5'- --aGCGCGGCGCGgCGugguaaCGGGcgagccGGUGCg -3' miRNA: 3'- uugUGUGCCGCGU-GUua----GCCU------UCACG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 45059 | 0.66 | 0.972291 |
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Target: 5'- aGAC-CACGGCGCuuguugGCuucUCGGAAGgagaagaUGCg -3' miRNA: 3'- -UUGuGUGCCGCG------UGuu-AGCCUUC-------ACG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 80490 | 0.66 | 0.965403 |
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Target: 5'- uGCGCcCGGCGgcgguuacacuuucCGCAAUgGGAAG-GCa -3' miRNA: 3'- uUGUGuGCCGC--------------GUGUUAgCCUUCaCG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 11121 | 0.66 | 0.959316 |
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Target: 5'- aAACACAaGGUGCGCGGUgGGAAccugaaGCa -3' miRNA: 3'- -UUGUGUgCCGCGUGUUAgCCUUca----CG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 44818 | 0.66 | 0.959316 |
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Target: 5'- uGCACGCGGCGgcaaacgcuCGCAuggUGGugucAAGUGCu -3' miRNA: 3'- uUGUGUGCCGC---------GUGUua-GCC----UUCACG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 77860 | 0.68 | 0.914412 |
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Target: 5'- uAGgAUACGGCGCGgGcuggaauAUCGGucAGGUGCu -3' miRNA: 3'- -UUgUGUGCCGCGUgU-------UAGCC--UUCACG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 127252 | 0.7 | 0.841798 |
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Target: 5'- cGCAUACGGCGCGgcGUCGGAcacgaaGCa -3' miRNA: 3'- uUGUGUGCCGCGUguUAGCCUuca---CG- -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 126125 | 0.71 | 0.800765 |
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Target: 5'- uGGCGCAugccCGGCGCACugAcaacuauggcuucugGUCGGAAGUGg -3' miRNA: 3'- -UUGUGU----GCCGCGUG--U---------------UAGCCUUCACg -5' |
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| 21028 | 5' | -52.4 | NC_004735.1 | + | 126810 | 1.09 | 0.004772 |
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Target: 5'- gAACACACGGCGCACAAUCGGAAGUGCc -3' miRNA: 3'- -UUGUGUGCCGCGUGUUAGCCUUCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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