Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21029 | 3' | -59.2 | NC_004735.1 | + | 126657 | 0.66 | 0.751838 |
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Target: 5'- aGCGGUGCGGUGUacuauguggaugugcCGGuuGCGucGCu -3' miRNA: 3'- aUGCCGCGCCGCA---------------GCCugUGCuuCGu -5' |
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| 21029 | 3' | -59.2 | NC_004735.1 | + | 29398 | 0.66 | 0.748016 |
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Target: 5'- aUACuGGCGCaccaGCGcuUCGG-CACGAAGCc -3' miRNA: 3'- -AUG-CCGCGc---CGC--AGCCuGUGCUUCGu -5' |
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| 21029 | 3' | -59.2 | NC_004735.1 | + | 129886 | 0.66 | 0.738391 |
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Target: 5'- gUACGGCugGCGGUccGUCGGugGgaCGGucAGCAa -3' miRNA: 3'- -AUGCCG--CGCCG--CAGCCugU--GCU--UCGU- -5' |
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| 21029 | 3' | -59.2 | NC_004735.1 | + | 82421 | 0.66 | 0.712963 |
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Target: 5'- gUugGGCuuGGaGUCGGACACGAuuuacuucuccaauaAGCu -3' miRNA: 3'- -AugCCGcgCCgCAGCCUGUGCU---------------UCGu -5' |
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| 21029 | 3' | -59.2 | NC_004735.1 | + | 28995 | 0.74 | 0.286678 |
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Target: 5'- cGCGGCGCGGCGUgguaaCGGGCGagcCGguGCGu -3' miRNA: 3'- aUGCCGCGCCGCA-----GCCUGU---GCuuCGU- -5' |
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| 21029 | 3' | -59.2 | NC_004735.1 | + | 127255 | 1.07 | 0.001695 |
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Target: 5'- aUACGGCGCGGCGUCGGACACGAAGCAg -3' miRNA: 3'- -AUGCCGCGCCGCAGCCUGUGCUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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