Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21029 | 5' | -56.7 | NC_004735.1 | + | 45755 | 0.66 | 0.852562 |
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Target: 5'- --cCCUCCGUCUCaguucggcgCUgACCAuGCaaCACCg -3' miRNA: 3'- aaaGGAGGCAGAG---------GAgUGGU-CG--GUGG- -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 27577 | 0.66 | 0.843698 |
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Target: 5'- -cUCCUcgcggucugaucaCCGUaacCUCCUgACCAcCCACCa -3' miRNA: 3'- aaAGGA-------------GGCA---GAGGAgUGGUcGGUGG- -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 39797 | 0.66 | 0.82783 |
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Target: 5'- -cUCCUCC-UUUCgaUUCACCAGCgACUc -3' miRNA: 3'- aaAGGAGGcAGAG--GAGUGGUCGgUGG- -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 30795 | 0.66 | 0.819211 |
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Target: 5'- uUUUCCUCCGUUUCC-CugaUAGCgGCa -3' miRNA: 3'- -AAAGGAGGCAGAGGaGug-GUCGgUGg -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 88916 | 0.67 | 0.810418 |
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Target: 5'- -gUUUUCUGUUUCCUCccGCCGGUUACa -3' miRNA: 3'- aaAGGAGGCAGAGGAG--UGGUCGGUGg -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 95621 | 0.7 | 0.590368 |
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Target: 5'- aUUCCUCCGUUUCaUCagugcaaGCCAGCUugUa -3' miRNA: 3'- aAAGGAGGCAGAGgAG-------UGGUCGGugG- -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 508 | 0.72 | 0.494906 |
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Target: 5'- -aUCCUCCGUCUCCcCAcucuucuugaaaaacCCGGCgGCa -3' miRNA: 3'- aaAGGAGGCAGAGGaGU---------------GGUCGgUGg -5' |
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| 21029 | 5' | -56.7 | NC_004735.1 | + | 127291 | 1.09 | 0.001824 |
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Target: 5'- aUUUCCUCCGUCUCCUCACCAGCCACCu -3' miRNA: 3'- -AAAGGAGGCAGAGGAGUGGUCGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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