Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21035 | 3' | -58.7 | NC_004745.1 | + | 23467 | 0.66 | 0.37683 |
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Target: 5'- aAAUGGCUugcGAAUGUaUGCCGCaggaUGUGGGu -3' miRNA: 3'- -UUGCCGA---CUUGUA-GCGGCGg---ACGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 922 | 0.67 | 0.32284 |
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Target: 5'- aAGCGGCUGAA-AUC-CUGCUgggaaagccacgggUGCGGGa -3' miRNA: 3'- -UUGCCGACUUgUAGcGGCGG--------------ACGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 2908 | 0.67 | 0.291668 |
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Target: 5'- uGCGGCacgGAaaaccccugccaguACAgcagCGCCGCCUgacgacGCGGGu -3' miRNA: 3'- uUGCCGa--CU--------------UGUa---GCGGCGGA------CGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 22257 | 0.68 | 0.251391 |
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Target: 5'- uGAUGGCg----GUCGCCGUcgCUGCGGGu -3' miRNA: 3'- -UUGCCGacuugUAGCGGCG--GACGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 6113 | 0.69 | 0.219935 |
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Target: 5'- -cCGGCU---CGUCGCCuGCCgguUGCGGGa -3' miRNA: 3'- uuGCCGAcuuGUAGCGG-CGG---ACGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 27735 | 0.69 | 0.214062 |
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Target: 5'- -cCGGUauUGAGCGUuaCGCCGCCgcagagggugaUGUGGGg -3' miRNA: 3'- uuGCCG--ACUUGUA--GCGGCGG-----------ACGCCC- -5' |
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| 21035 | 3' | -58.7 | NC_004745.1 | + | 228 | 1.07 | 0.000283 |
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Target: 5'- cAACGGCUGAACAUCGCCGCCUGCGGGc -3' miRNA: 3'- -UUGCCGACUUGUAGCGGCGGACGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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