Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21037 | 3' | -50.9 | NC_004745.1 | + | 3999 | 0.66 | 0.785448 |
Target: 5'- --gCCCUgaugacgaacUGCUGACCGGCg -3' miRNA: 3'- aaaGGGGaaaguaaa--GCGACUGGCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 24142 | 0.66 | 0.782218 |
Target: 5'- -cUCCCCaUUC-UUUC--UGACUGGCa -3' miRNA: 3'- aaAGGGGaAAGuAAAGcgACUGGCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 18170 | 0.66 | 0.771337 |
Target: 5'- uUUUCaUCCgggUCAgccUgGCUGACCGGUu -3' miRNA: 3'- -AAAG-GGGaa-AGUaa-AgCGACUGGCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 27079 | 0.67 | 0.691245 |
Target: 5'- -aUCCaCUUUUCAgcgUCGUuuaUGGCCGcGCa -3' miRNA: 3'- aaAGG-GGAAAGUaa-AGCG---ACUGGC-CG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 12046 | 0.69 | 0.572178 |
Target: 5'- --aCCUCaugcaccaGUUUCGuCUGACCGGCa -3' miRNA: 3'- aaaGGGGaaag----UAAAGC-GACUGGCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 7515 | 0.69 | 0.568638 |
Target: 5'- --aUCCCUUUCAcugaugcugaacgugUCGCUGgcgcugauGCCGGCa -3' miRNA: 3'- aaaGGGGAAAGUaa-------------AGCGAC--------UGGCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 407 | 0.71 | 0.447768 |
Target: 5'- -aUCCCCUUUCA----GUUGAUgGGCg -3' miRNA: 3'- aaAGGGGAAAGUaaagCGACUGgCCG- -5' |
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21037 | 3' | -50.9 | NC_004745.1 | + | 4307 | 1.11 | 0.000862 |
Target: 5'- uUUUCCCCUUUCAUUUCGCUGACCGGCa -3' miRNA: 3'- -AAAGGGGAAAGUAAAGCGACUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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