Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21038 | 3' | -54.1 | NC_004745.1 | + | 18541 | 0.66 | 0.554063 |
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Target: 5'- gAUGUgGCAGUGauauuucacUGGCCGcCAUCAGAAc -3' miRNA: 3'- -UGCGgUGUCGU---------ACUGGCaGUAGUCUU- -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 20989 | 0.67 | 0.519814 |
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Target: 5'- uGCGCCggggacACAGCuUGACCGcCAguuaCAGGAu -3' miRNA: 3'- -UGCGG------UGUCGuACUGGCaGUa---GUCUU- -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 26903 | 0.68 | 0.464745 |
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Target: 5'- cGCGCCAC-GCGaGGCCGUCGggCGcGAAa -3' miRNA: 3'- -UGCGGUGuCGUaCUGGCAGUa-GU-CUU- -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 19529 | 0.69 | 0.364727 |
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Target: 5'- aGCGCCAuauuCAGCGUGACCGgCGUUg--- -3' miRNA: 3'- -UGCGGU----GUCGUACUGGCaGUAGucuu -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 4057 | 0.72 | 0.258564 |
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Target: 5'- gACGCCGguCAGCAgu-UCGUCAUCAGGg -3' miRNA: 3'- -UGCGGU--GUCGUacuGGCAGUAGUCUu -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 909 | 0.74 | 0.195204 |
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Target: 5'- aGCGCUACAGCAccACCGguaaggUCAUCAGAc -3' miRNA: 3'- -UGCGGUGUCGUacUGGC------AGUAGUCUu -5' |
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| 21038 | 3' | -54.1 | NC_004745.1 | + | 5457 | 1.06 | 0.000824 |
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Target: 5'- gACGCCACAGCAUGACCGUCAUCAGAAu -3' miRNA: 3'- -UGCGGUGUCGUACUGGCAGUAGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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