Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21039 | 3' | -55.4 | NC_004745.1 | + | 5856 | 0.66 | 0.511761 |
Target: 5'- -aCACCG-GCAaaACGUGgaCGCCCGAa-- -3' miRNA: 3'- ugGUGGCuCGU--UGUAC--GCGGGCUauu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 9520 | 0.66 | 0.478917 |
Target: 5'- cGCUGCCGAgugcagaccgGCGGCAUGUGCaCUGAc-- -3' miRNA: 3'- -UGGUGGCU----------CGUUGUACGCG-GGCUauu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 19089 | 0.66 | 0.468199 |
Target: 5'- aACCACCGcacagcuuaaccGGCAGCGUGaCGCgCUGGc-- -3' miRNA: 3'- -UGGUGGC------------UCGUUGUAC-GCG-GGCUauu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 6518 | 0.66 | 0.454453 |
Target: 5'- cGCUACaGGGCGACAcgcucgacgccauuUGUGUCCGGUAu -3' miRNA: 3'- -UGGUGgCUCGUUGU--------------ACGCGGGCUAUu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 19389 | 0.67 | 0.416574 |
Target: 5'- uACCGCCGccucGGCggAugAUGCGgCCGGUGc -3' miRNA: 3'- -UGGUGGC----UCG--UugUACGCgGGCUAUu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 3825 | 0.68 | 0.396919 |
Target: 5'- cGCCACUGAGCAGCGccuCGCUgaGAUGGa -3' miRNA: 3'- -UGGUGGCUCGUUGUac-GCGGg-CUAUU- -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 27089 | 0.68 | 0.396919 |
Target: 5'- -gCACCGGGCuuuuauUGCGCCgGAUGu -3' miRNA: 3'- ugGUGGCUCGuugu--ACGCGGgCUAUu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 21391 | 0.68 | 0.387318 |
Target: 5'- uGCCAUCcAGCAACGgccaCGCCCGAc-- -3' miRNA: 3'- -UGGUGGcUCGUUGUac--GCGGGCUauu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 1771 | 0.68 | 0.387318 |
Target: 5'- -aCACCGuGGCGACA-GUGCCgGAUGc -3' miRNA: 3'- ugGUGGC-UCGUUGUaCGCGGgCUAUu -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 25270 | 0.68 | 0.350472 |
Target: 5'- aACaGCC-AGCAGCuAUGCGCCgGAUAAu -3' miRNA: 3'- -UGgUGGcUCGUUG-UACGCGGgCUAUU- -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 9340 | 0.68 | 0.350472 |
Target: 5'- cACCggugaaGCCGAGCAGagggcGCGCCgGAUAAu -3' miRNA: 3'- -UGG------UGGCUCGUUgua--CGCGGgCUAUU- -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 8843 | 0.69 | 0.324516 |
Target: 5'- cGCCGCCgGAGCcgguCAccCGCCCGAUGGa -3' miRNA: 3'- -UGGUGG-CUCGuu--GUacGCGGGCUAUU- -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 3978 | 0.7 | 0.284495 |
Target: 5'- gGCCACCGgcGGUGGgGUGaCGCCCuGAUGAc -3' miRNA: 3'- -UGGUGGC--UCGUUgUAC-GCGGG-CUAUU- -5' |
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21039 | 3' | -55.4 | NC_004745.1 | + | 6870 | 1.06 | 0.000606 |
Target: 5'- aACCACCGAGCAACAUGCGCCCGAUAAa -3' miRNA: 3'- -UGGUGGCUCGUUGUACGCGGGCUAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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