Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21039 | 5' | -54.8 | NC_004745.1 | + | 4943 | 0.66 | 0.575011 |
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Target: 5'- -----cGGGacgcgUACCGCCGGCAGGUu -3' miRNA: 3'- ccacuaCCCaa---GUGGUGGCCGUUCGu -5' |
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| 21039 | 5' | -54.8 | NC_004745.1 | + | 10023 | 0.68 | 0.46482 |
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Target: 5'- -uUGAUGaGaGUUCACCGCCGGUguGUu -3' miRNA: 3'- ccACUAC-C-CAAGUGGUGGCCGuuCGu -5' |
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| 21039 | 5' | -54.8 | NC_004745.1 | + | 9909 | 0.72 | 0.26974 |
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Target: 5'- gGGUGAcgcGGGUUgaugcuuucaccauaACCACCGGCAccguGGCAg -3' miRNA: 3'- -CCACUa--CCCAAg--------------UGGUGGCCGU----UCGU- -5' |
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| 21039 | 5' | -54.8 | NC_004745.1 | + | 3529 | 0.73 | 0.210411 |
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Target: 5'- -cUGGUGGGgcuggccgUCACCGaugacCCGGCAAGCc -3' miRNA: 3'- ccACUACCCa-------AGUGGU-----GGCCGUUCGu -5' |
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| 21039 | 5' | -54.8 | NC_004745.1 | + | 6037 | 0.74 | 0.198978 |
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Target: 5'- cGGUGA-GGGgacgguggUCAUCccgcaACCGGCAGGCGa -3' miRNA: 3'- -CCACUaCCCa-------AGUGG-----UGGCCGUUCGU- -5' |
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| 21039 | 5' | -54.8 | NC_004745.1 | + | 6834 | 1.1 | 0.000403 |
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Target: 5'- gGGUGAUGGGUUCACCACCGGCAAGCAc -3' miRNA: 3'- -CCACUACCCAAGUGGUGGCCGUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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