Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21040 | 5' | -58.8 | NC_004745.1 | + | 5508 | 0.66 | 0.340394 |
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Target: 5'- -cGCCGCACcCCGCcguacauguucaccgAGGaGGUGGCGc -3' miRNA: 3'- uaCGGCGUGuGGCG---------------UCCgUCACUGUc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 10851 | 0.66 | 0.336994 |
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Target: 5'- -gGCCGCAgUGCCGaCGGGCGuGUcGCGGa -3' miRNA: 3'- uaCGGCGU-GUGGC-GUCCGU-CAcUGUC- -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 167 | 0.66 | 0.328604 |
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Target: 5'- -aGCCGC---CCGCAGGCGGcGAUg- -3' miRNA: 3'- uaCGGCGuguGGCGUCCGUCaCUGuc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 22247 | 0.68 | 0.253208 |
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Target: 5'- -cGCCGU-CGCUGCGGGUuauGGUGACc- -3' miRNA: 3'- uaCGGCGuGUGGCGUCCG---UCACUGuc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 1551 | 0.68 | 0.246505 |
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Target: 5'- -cGCCG-ACACCG-AGGCcGGUGGCAu -3' miRNA: 3'- uaCGGCgUGUGGCgUCCG-UCACUGUc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 26504 | 0.69 | 0.215177 |
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Target: 5'- -aGCCGCACGacgggcaGCAGGCAuUGGCGu -3' miRNA: 3'- uaCGGCGUGUgg-----CGUCCGUcACUGUc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 19771 | 0.69 | 0.209336 |
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Target: 5'- cGUGCCGUGuuaagcCGCCuGCAGGCAccGACGGg -3' miRNA: 3'- -UACGGCGU------GUGG-CGUCCGUcaCUGUC- -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 9624 | 0.69 | 0.187325 |
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Target: 5'- gGUGCUGauuCugGCCGUGGGCGGUGAa-- -3' miRNA: 3'- -UACGGC---GugUGGCGUCCGUCACUguc -5' |
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| 21040 | 5' | -58.8 | NC_004745.1 | + | 6941 | 1.07 | 0.000209 |
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Target: 5'- gAUGCCGCACACCGCAGGCAGUGACAGg -3' miRNA: 3'- -UACGGCGUGUGGCGUCCGUCACUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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