Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21042 | 5' | -54.4 | NC_004745.1 | + | 8244 | 0.66 | 0.571985 |
Target: 5'- cUGuGCGCCGGUUGCAc---CAGCGcCc -3' miRNA: 3'- uGC-CGUGGCCGACGUuuuuGUCGCuG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 10705 | 0.66 | 0.560456 |
Target: 5'- -aGGUcauuACCGGCUGCAcAGGcCAGCa-- -3' miRNA: 3'- ugCCG----UGGCCGACGUuUUU-GUCGcug -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 8647 | 0.66 | 0.548991 |
Target: 5'- cCGGUGCUggcguGGCUGCGGGAAaauCAGCccGACa -3' miRNA: 3'- uGCCGUGG-----CCGACGUUUUU---GUCG--CUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 16711 | 0.66 | 0.537601 |
Target: 5'- gACGGCACCGGCgacGacgaGGAAACGaaacucgcGCaGACu -3' miRNA: 3'- -UGCCGUGGCCGa--Cg---UUUUUGU--------CG-CUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 7452 | 0.66 | 0.537601 |
Target: 5'- cCGuaACCGGCacccgGCAGGGACGcccagauauuGCGACa -3' miRNA: 3'- uGCcgUGGCCGa----CGUUUUUGU----------CGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 18394 | 0.66 | 0.526293 |
Target: 5'- gACGGCACCGauGCUGaccGAGCAG-GAg -3' miRNA: 3'- -UGCCGUGGC--CGACguuUUUGUCgCUg -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 21355 | 0.67 | 0.515076 |
Target: 5'- gAUGGCACCGGCaUGauuu-ACGGCa-- -3' miRNA: 3'- -UGCCGUGGCCG-ACguuuuUGUCGcug -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 2067 | 0.67 | 0.492943 |
Target: 5'- gGCGGCGCUGGC-GCGuccccgguuGAACAGUucacccGACc -3' miRNA: 3'- -UGCCGUGGCCGaCGUu--------UUUGUCG------CUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 2975 | 0.67 | 0.471257 |
Target: 5'- gGCGGCGCU-GCUGUAcuGGCAGgGGu -3' miRNA: 3'- -UGCCGUGGcCGACGUuuUUGUCgCUg -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 5707 | 0.68 | 0.460597 |
Target: 5'- -gGGCGCUGGC-GCAccugcAACGuGCGACa -3' miRNA: 3'- ugCCGUGGCCGaCGUuu---UUGU-CGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 15145 | 0.68 | 0.439668 |
Target: 5'- uGCGGCAuuuCCGGCcauucaggauuUGCAGGAuccacACGGCuGACc -3' miRNA: 3'- -UGCCGU---GGCCG-----------ACGUUUU-----UGUCG-CUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 19215 | 0.69 | 0.3995 |
Target: 5'- -gGGCgcuGCCGGUgugGgGAuuGCGGCGGCg -3' miRNA: 3'- ugCCG---UGGCCGa--CgUUuuUGUCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 1123 | 0.69 | 0.380315 |
Target: 5'- cCGGCACCGGCUcGCcAAA--GGUGAa -3' miRNA: 3'- uGCCGUGGCCGA-CGuUUUugUCGCUg -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 11145 | 0.7 | 0.343836 |
Target: 5'- cACaGCACCGGCUGgG---GC-GCGACa -3' miRNA: 3'- -UGcCGUGGCCGACgUuuuUGuCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 22253 | 0.7 | 0.343836 |
Target: 5'- gGCGGuCGCCGucGCUGCGGGuuAUGGUGACc -3' miRNA: 3'- -UGCC-GUGGC--CGACGUUUu-UGUCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 20853 | 0.7 | 0.335118 |
Target: 5'- uAUGGCACCGGUgGUuauauucCGGCGACc -3' miRNA: 3'- -UGCCGUGGCCGaCGuuuuu--GUCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 7923 | 0.7 | 0.309944 |
Target: 5'- gACGGCagGCCGuacauaucaGCcGgAAGAACAGCGACg -3' miRNA: 3'- -UGCCG--UGGC---------CGaCgUUUUUGUCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 19455 | 0.7 | 0.309944 |
Target: 5'- cUGuGCACCGGCcGCAucauccgccGAGGCGGCGGu -3' miRNA: 3'- uGC-CGUGGCCGaCGU---------UUUUGUCGCUg -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 7750 | 0.72 | 0.263979 |
Target: 5'- cCGGUGCCguGGC-GCAGAcgGGCGGCGGCu -3' miRNA: 3'- uGCCGUGG--CCGaCGUUU--UUGUCGCUG- -5' |
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21042 | 5' | -54.4 | NC_004745.1 | + | 4578 | 0.72 | 0.249936 |
Target: 5'- gGCGGUcgGCUGGCUGCAGAAAUAccGCa-- -3' miRNA: 3'- -UGCCG--UGGCCGACGUUUUUGU--CGcug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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