Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21043 | 3' | -60.5 | NC_004745.1 | + | 1164 | 0.66 | 0.290472 |
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Target: 5'- aGCCGGUgccgGUACUCGaCCGCCguGACa -3' miRNA: 3'- gCGGUCGaga-CGUGAGUcGGCGG--CUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 7620 | 0.66 | 0.283121 |
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Target: 5'- gGCCAcGCUCUGCGgguaaaUCAGggacagUGCCGGCa -3' miRNA: 3'- gCGGU-CGAGACGUg-----AGUCg-----GCGGCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 20267 | 0.67 | 0.229557 |
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Target: 5'- uGCCGGCggUGCACUgGuGCUGaCUGGCa -3' miRNA: 3'- gCGGUCGagACGUGAgU-CGGC-GGCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 1577 | 0.67 | 0.229557 |
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Target: 5'- -uCCAGCUCgUGCGCUCGuucuauccuGCCGCgcgCGAUa -3' miRNA: 3'- gcGGUCGAG-ACGUGAGU---------CGGCG---GCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 20325 | 0.68 | 0.206134 |
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Target: 5'- uGCCAG-UCaGCAC-CAGugcaCCGCCGGCa -3' miRNA: 3'- gCGGUCgAGaCGUGaGUC----GGCGGCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 19371 | 0.68 | 0.195217 |
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Target: 5'- gCGCCAGCUCgGCGacaauaccGCCGCCucGGCg -3' miRNA: 3'- -GCGGUCGAGaCGUgagu----CGGCGG--CUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 9138 | 0.69 | 0.17979 |
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Target: 5'- aCGaCCGGUcuuuuuUCUGgCGCUCuGCUGCUGGCg -3' miRNA: 3'- -GC-GGUCG------AGAC-GUGAGuCGGCGGCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 20280 | 0.74 | 0.074114 |
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Target: 5'- aCGCCAGUgauuuguuuuUCUGUAUcCAGCCGUCGAg -3' miRNA: 3'- -GCGGUCG----------AGACGUGaGUCGGCGGCUg -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 9572 | 0.78 | 0.033235 |
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Target: 5'- cCGCCGG-UCUGCACUCGGCagCGaCCGGCg -3' miRNA: 3'- -GCGGUCgAGACGUGAGUCG--GC-GGCUG- -5' |
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| 21043 | 3' | -60.5 | NC_004745.1 | + | 9031 | 1.09 | 0.000109 |
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Target: 5'- uCGCCAGCUCUGCACUCAGCCGCCGACg -3' miRNA: 3'- -GCGGUCGAGACGUGAGUCGGCGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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