Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21044 | 3' | -57.9 | NC_004745.1 | + | 167 | 0.66 | 0.349884 |
Target: 5'- --aGCCGCCCGCaGGCgGcGAuguUCAGCc -3' miRNA: 3'- aagUGGCGGGUG-CCGgU-CUu--AGUCGu -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 2048 | 0.67 | 0.324337 |
Target: 5'- cUCugUGCggACGGCCAGuggCGGCAg -3' miRNA: 3'- aAGugGCGggUGCCGGUCuuaGUCGU- -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 9979 | 0.68 | 0.284153 |
Target: 5'- gUC-CCGCCCuucugcacuuccaGCGuGCCGGuAAUCAGCc -3' miRNA: 3'- aAGuGGCGGG-------------UGC-CGGUC-UUAGUCGu -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 9001 | 0.68 | 0.277484 |
Target: 5'- --aAgCGUCUGCGG-CAGAGUCAGCAg -3' miRNA: 3'- aagUgGCGGGUGCCgGUCUUAGUCGU- -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 16511 | 0.68 | 0.277484 |
Target: 5'- -cCACCGUauCCACuGCCAuuGUCGGCAu -3' miRNA: 3'- aaGUGGCG--GGUGcCGGUcuUAGUCGU- -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 5398 | 0.69 | 0.211616 |
Target: 5'- -aCGCCGuucaCCCACGGCaacCAGAAcggCAGCAg -3' miRNA: 3'- aaGUGGC----GGGUGCCG---GUCUUa--GUCGU- -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 6888 | 0.71 | 0.173903 |
Target: 5'- gUCACUGCCUGCGGUgugCGGcAUCGGCu -3' miRNA: 3'- aAGUGGCGGGUGCCG---GUCuUAGUCGu -5' |
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21044 | 3' | -57.9 | NC_004745.1 | + | 9682 | 1.06 | 0.000346 |
Target: 5'- gUUCACCGCCCACGGCCAGAAUCAGCAc -3' miRNA: 3'- -AAGUGGCGGGUGCCGGUCUUAGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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