Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21045 | 5' | -61.5 | NC_004745.1 | + | 21111 | 0.66 | 0.304387 |
Target: 5'- -uGCAUCaUGCUGGCaCGGGCGcGCGCa -3' miRNA: 3'- ccCGUAG-GCGGCUGcGUCUGC-CGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 1289 | 0.66 | 0.289697 |
Target: 5'- aGGGCGUgcauggUCGCCcaGGCGaGGuCGGCGUGg -3' miRNA: 3'- -CCCGUA------GGCGG--CUGCgUCuGCCGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 2032 | 0.66 | 0.289697 |
Target: 5'- aGGGCGUUaugacugaCGUCGAuuuccaCGCGuucGGCGGCGCu -3' miRNA: 3'- -CCCGUAG--------GCGGCU------GCGU---CUGCCGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 8093 | 0.66 | 0.279748 |
Target: 5'- aGGGCAgacaaaaacgaGCUGGCGCAGGUGGCacuGCGu -3' miRNA: 3'- -CCCGUagg--------CGGCUGCGUCUGCCG---CGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 9337 | 0.66 | 0.275568 |
Target: 5'- cGGUGaa-GCCGA-GCAGAgGGCGCGc -3' miRNA: 3'- cCCGUaggCGGCUgCGUCUgCCGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 9516 | 0.66 | 0.261996 |
Target: 5'- gGGGCGUaucaUGuuG-CGCAGuGCGcGCGCGa -3' miRNA: 3'- -CCCGUAg---GCggCuGCGUC-UGC-CGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 21758 | 0.67 | 0.242041 |
Target: 5'- cGGGCAggucgggCUGCCGguucgugGCGCuguccuGACGGUGUa -3' miRNA: 3'- -CCCGUa------GGCGGC-------UGCGu-----CUGCCGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 19328 | 0.68 | 0.218181 |
Target: 5'- uGGCAaaagacUCCGCCGAaaugaccgcacuaCGCAaACaGGCGCGc -3' miRNA: 3'- cCCGU------AGGCGGCU-------------GCGUcUG-CCGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 7751 | 0.68 | 0.213098 |
Target: 5'- cGGUG-CCG-UGGCGCAGACGG-GCGg -3' miRNA: 3'- cCCGUaGGCgGCUGCGUCUGCCgCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 19214 | 0.68 | 0.213098 |
Target: 5'- uGGGCG-CUGCCGGuguggggauUGCGG-CGGCGgGa -3' miRNA: 3'- -CCCGUaGGCGGCU---------GCGUCuGCCGCgC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 5641 | 0.68 | 0.213098 |
Target: 5'- aGGGUGUCCGUCa--GCAGGCGGgGa- -3' miRNA: 3'- -CCCGUAGGCGGcugCGUCUGCCgCgc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 8971 | 0.68 | 0.196882 |
Target: 5'- aGGGCGUCggCGCUGAgUGCAGAgcUGGCGa- -3' miRNA: 3'- -CCCGUAG--GCGGCU-GCGUCU--GCCGCgc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 4033 | 0.68 | 0.196882 |
Target: 5'- aGGGCGUcaccgccaCCGCCgGugGCcuugcuGCGGCGCu -3' miRNA: 3'- -CCCGUA--------GGCGG-CugCGuc----UGCCGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 11434 | 0.68 | 0.191721 |
Target: 5'- cGGCAaaucugCUGCCGugGCuggcGugGGCGUu -3' miRNA: 3'- cCCGUa-----GGCGGCugCGu---CugCCGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 19447 | 0.7 | 0.160557 |
Target: 5'- cGGCcgcaucAUCCGCCGAgGCGGcgguauugucgccgaGCuGGCGCGc -3' miRNA: 3'- cCCG------UAGGCGGCUgCGUC---------------UG-CCGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 11552 | 0.7 | 0.154562 |
Target: 5'- aGGCA-CUGUCGGCGCAaucCGGCGUGu -3' miRNA: 3'- cCCGUaGGCGGCUGCGUcu-GCCGCGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 9021 | 0.7 | 0.154562 |
Target: 5'- -uGCAcucagCCGCCGACGCccuGACGGUgacaGCGa -3' miRNA: 3'- ccCGUa----GGCGGCUGCGu--CUGCCG----CGC- -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 20258 | 0.7 | 0.150405 |
Target: 5'- cGGCAUCauuGCCGGCGguGcACuGGUGCu -3' miRNA: 3'- cCCGUAGg--CGGCUGCguC-UG-CCGCGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 9516 | 0.7 | 0.142394 |
Target: 5'- cGGUcgCUGCCGAgUGCAGAcCGGCgGCa -3' miRNA: 3'- cCCGuaGGCGGCU-GCGUCU-GCCG-CGc -5' |
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21045 | 5' | -61.5 | NC_004745.1 | + | 5961 | 0.71 | 0.131108 |
Target: 5'- cGGCGUgCCGCCcuGGCGCGG--GGCGCa -3' miRNA: 3'- cCCGUA-GGCGG--CUGCGUCugCCGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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