Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21046 | 3' | -52.7 | NC_004745.1 | + | 9543 | 0.66 | 0.682142 |
Target: 5'- gUUAAGGUCGguuucgacgauaaUGCCGGggcguaucaugUUGCGCAGuGCg -3' miRNA: 3'- -AGUUCCAGUa------------AUGGCC-----------GACGUGUC-CG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 11763 | 0.66 | 0.675142 |
Target: 5'- aUCcAGGaCAUUcCCGGCuaucUGUauACAGGCg -3' miRNA: 3'- -AGuUCCaGUAAuGGCCG----ACG--UGUCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 27343 | 0.66 | 0.663437 |
Target: 5'- ---uGGUCAUguUGCUGGCgcUGCACGaGCu -3' miRNA: 3'- aguuCCAGUA--AUGGCCG--ACGUGUcCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 20688 | 0.67 | 0.628174 |
Target: 5'- aCGGGcGUCAUguuCgGGCagGCACuGGCu -3' miRNA: 3'- aGUUC-CAGUAau-GgCCGa-CGUGuCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 21331 | 0.67 | 0.628174 |
Target: 5'- -gAAGGUCgggcguggccGUUGCUGGaugGCACcGGCa -3' miRNA: 3'- agUUCCAG----------UAAUGGCCga-CGUGuCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 8336 | 0.67 | 0.592944 |
Target: 5'- gCAGGcGUUGUggacaaucACUGGCgucUGCGCAGGCc -3' miRNA: 3'- aGUUC-CAGUAa-------UGGCCG---ACGUGUCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 5880 | 0.67 | 0.581262 |
Target: 5'- cUCcGGGUC-UUACgCGGg-GCGCGGGUg -3' miRNA: 3'- -AGuUCCAGuAAUG-GCCgaCGUGUCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 10608 | 0.7 | 0.406551 |
Target: 5'- aUCuGGcGUCAgcgugUGaaugaGGCUGCACGGGCg -3' miRNA: 3'- -AGuUC-CAGUa----AUgg---CCGACGUGUCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 18239 | 0.7 | 0.406551 |
Target: 5'- aUCGGGGUUA--ACCGGUcaGC-CAGGCu -3' miRNA: 3'- -AGUUCCAGUaaUGGCCGa-CGuGUCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 20256 | 0.73 | 0.283548 |
Target: 5'- aUCGGcaUCAUUGCCGGCggUGCACuGGUg -3' miRNA: 3'- -AGUUccAGUAAUGGCCG--ACGUGuCCG- -5' |
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21046 | 3' | -52.7 | NC_004745.1 | + | 10709 | 1.12 | 0.000473 |
Target: 5'- aUCAAGGUCAUUACCGGCUGCACAGGCc -3' miRNA: 3'- -AGUUCCAGUAAUGGCCGACGUGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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