Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21047 | 3' | -62.5 | NC_004745.1 | + | 9696 | 0.66 | 0.21298 |
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Target: 5'- -cCCCGCGCCGuCUGCGuCGGCGGAUg -3' miRNA: 3'- gcGGGUGCGGUcGGUGCcGUCGUCUA- -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 9157 | 0.66 | 0.191192 |
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Target: 5'- -aUCCGCGCCAGCC-CGGaacaGGCAu-- -3' miRNA: 3'- gcGGGUGCGGUCGGuGCCg---UCGUcua -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 19679 | 0.67 | 0.162185 |
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Target: 5'- uCGCUCAUGCC-GUCAaaauCGGCGGCGGu- -3' miRNA: 3'- -GCGGGUGCGGuCGGU----GCCGUCGUCua -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 1363 | 0.69 | 0.12969 |
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Target: 5'- gCGUCCGCuGCUGGUCAUGGUcuuGCGGAUa -3' miRNA: 3'- -GCGGGUG-CGGUCGGUGCCGu--CGUCUA- -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 17032 | 0.69 | 0.12969 |
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Target: 5'- aGCUCACGCUGGCUGaaauucUGGCGGUAGGc -3' miRNA: 3'- gCGGGUGCGGUCGGU------GCCGUCGUCUa -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 22988 | 0.69 | 0.113836 |
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Target: 5'- aGUCCACGCCguggGGCCGCacccguugccgucagGGCAGCAa-- -3' miRNA: 3'- gCGGGUGCGG----UCGGUG---------------CCGUCGUcua -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 8697 | 0.7 | 0.103301 |
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Target: 5'- cCGCagCCACGCCAGCaC-CGGCacaaugAGCAGGUc -3' miRNA: 3'- -GCG--GGUGCGGUCG-GuGCCG------UCGUCUA- -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 28303 | 0.7 | 0.09754 |
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Target: 5'- aCGCCCAUGCgguaaCAGCCGCaGCAGUAu-- -3' miRNA: 3'- -GCGGGUGCG-----GUCGGUGcCGUCGUcua -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 23826 | 0.71 | 0.082028 |
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Target: 5'- gGCCUcCGCCAGUgGCGGCAGUccgugaauuGGGUg -3' miRNA: 3'- gCGGGuGCGGUCGgUGCCGUCG---------UCUA- -5' |
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| 21047 | 3' | -62.5 | NC_004745.1 | + | 11496 | 1.05 | 0.00014 |
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Target: 5'- aCGCCCACGCCAGCCACGGCAGCAGAUu -3' miRNA: 3'- -GCGGGUGCGGUCGGUGCCGUCGUCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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