Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21049 | 3' | -54.1 | NC_004745.1 | + | 164 | 0.66 | 0.607077 |
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Target: 5'- aAAAGCCGcCcgcagGCGGCGAuguUCAGcCGUUGu -3' miRNA: 3'- -UUUUGGCaG-----CGCCGCUu--GGUC-GCAAC- -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 9635 | 0.66 | 0.58388 |
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Target: 5'- -uGGCCGUgGgCGGUGAACUcgacacGGCGUUc -3' miRNA: 3'- uuUUGGCAgC-GCCGCUUGG------UCGCAAc -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 19548 | 0.66 | 0.572344 |
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Target: 5'- --cAUCGU-GCGGCGGuucGCCAGCGc-- -3' miRNA: 3'- uuuUGGCAgCGCCGCU---UGGUCGCaac -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 8304 | 0.66 | 0.572344 |
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Target: 5'- cAGGCCGggCGCuGGUGcAACCGGCGc-- -3' miRNA: 3'- uUUUGGCa-GCG-CCGC-UUGGUCGCaac -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 617 | 0.68 | 0.482753 |
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Target: 5'- --cGCCGUuuucguaauacuUGCGGCGGAaCAGCGUg- -3' miRNA: 3'- uuuUGGCA------------GCGCCGCUUgGUCGCAac -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 17451 | 0.69 | 0.400479 |
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Target: 5'- --uGCCGUCaaugauGUcGCGAAUCAGCGUUGc -3' miRNA: 3'- uuuUGGCAG------CGcCGCUUGGUCGCAAC- -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 7186 | 0.69 | 0.390835 |
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Target: 5'- -uAACCGUCGCGGUGAAaaauccaCGGCc--- -3' miRNA: 3'- uuUUGGCAGCGCCGCUUg------GUCGcaac -5' |
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| 21049 | 3' | -54.1 | NC_004745.1 | + | 17292 | 1.06 | 0.000964 |
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Target: 5'- gAAAACCGUCGCGGCGAACCAGCGUUGu -3' miRNA: 3'- -UUUUGGCAGCGCCGCUUGGUCGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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