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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21050 | 3' | -52.6 | NC_004745.1 | + | 9103 | 0.67 | 0.614777 |
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Target: 5'- gUGGCGCgua--GGGugugCCGUCcgGGGCu -3' miRNA: 3'- gACCGCGaauugCCUua--GGCAG--UCCG- -5' |
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| 21050 | 3' | -52.6 | NC_004745.1 | + | 10866 | 0.69 | 0.54209 |
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Target: 5'- -gGGCGUgucGCGGAuaucucugucaccaGUCCGUCuccGGCc -3' miRNA: 3'- gaCCGCGaauUGCCU--------------UAGGCAGu--CCG- -5' |
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| 21050 | 3' | -52.6 | NC_004745.1 | + | 143 | 0.7 | 0.467134 |
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Target: 5'- -gGGCGUgaUGACGGGAUauaaaaaagccgcCCG-CAGGCg -3' miRNA: 3'- gaCCGCGa-AUUGCCUUA-------------GGCaGUCCG- -5' |
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| 21050 | 3' | -52.6 | NC_004745.1 | + | 5207 | 0.73 | 0.308022 |
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Target: 5'- gUGGCGCUguGCGGGAcUCGUCAucGGUu -3' miRNA: 3'- gACCGCGAauUGCCUUaGGCAGU--CCG- -5' |
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| 21050 | 3' | -52.6 | NC_004745.1 | + | 26885 | 0.76 | 0.195416 |
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Target: 5'- -cGGUGCUUAugcauggaacgcgccACGcGAggCCGUCGGGCg -3' miRNA: 3'- gaCCGCGAAU---------------UGC-CUuaGGCAGUCCG- -5' |
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| 21050 | 3' | -52.6 | NC_004745.1 | + | 19039 | 1.13 | 0.00047 |
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Target: 5'- gCUGGCGCUUAACGGAAUCCGUCAGGCg -3' miRNA: 3'- -GACCGCGAAUUGCCUUAGGCAGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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