Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21051 | 3' | -57.9 | NC_004745.1 | + | 6645 | 0.66 | 0.347701 |
Target: 5'- ---aGCCAguCCCGGauuuGCGGCgaGCAc -3' miRNA: 3'- aaaaCGGUguGGGUCg---UGCCGgaCGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 22540 | 0.66 | 0.347701 |
Target: 5'- ---cGCCugAgCgGcgcGCAUGGCCUGCGc -3' miRNA: 3'- aaaaCGGugUgGgU---CGUGCCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 11199 | 0.66 | 0.347701 |
Target: 5'- --aUGUCGCGcCCCAGC-CGGUgCUGUg -3' miRNA: 3'- aaaACGGUGU-GGGUCGuGCCG-GACGu -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 11939 | 0.66 | 0.330446 |
Target: 5'- ---gGCgGCugCCAGCuuuGCGGCUccgGCAg -3' miRNA: 3'- aaaaCGgUGugGGUCG---UGCCGGa--CGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 21398 | 0.66 | 0.330446 |
Target: 5'- --aUGCCGgugcCAUCCAGCaACGGCCacGCc -3' miRNA: 3'- aaaACGGU----GUGGGUCG-UGCCGGa-CGu -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 13815 | 0.66 | 0.319574 |
Target: 5'- ---cGCCGCAUugccggacaguucaCCAGagaGGCCUGCAc -3' miRNA: 3'- aaaaCGGUGUG--------------GGUCgugCCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 306 | 0.67 | 0.289342 |
Target: 5'- cUUUGCCACuuucucaauaucGCCCAGUGacccgacguucucCGGCUUGCc -3' miRNA: 3'- aAAACGGUG------------UGGGUCGU-------------GCCGGACGu -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 18437 | 0.68 | 0.253733 |
Target: 5'- --cUGCCugACCUuguGGCGCuGGCCgGUAa -3' miRNA: 3'- aaaACGGugUGGG---UCGUG-CCGGaCGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 18484 | 0.68 | 0.240266 |
Target: 5'- ---cGCCACAaggucaggcaguUCCAGCGCGGCgaccucCUGCu -3' miRNA: 3'- aaaaCGGUGU------------GGGUCGUGCCG------GACGu -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 8249 | 0.69 | 0.197778 |
Target: 5'- ---cGCCGguUGCaCCAGCGCccGGCCUGCGc -3' miRNA: 3'- aaaaCGGU--GUG-GGUCGUG--CCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 17065 | 0.7 | 0.186903 |
Target: 5'- --aUGCCAcCGCUCGugcGCugGGUCUGCGc -3' miRNA: 3'- aaaACGGU-GUGGGU---CGugCCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 20658 | 0.71 | 0.157385 |
Target: 5'- ----aCCACugCCA-CGCGGCCUGUAa -3' miRNA: 3'- aaaacGGUGugGGUcGUGCCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 16523 | 0.81 | 0.028875 |
Target: 5'- --cUGCCAUugUCGGCAUGGUCUGCAc -3' miRNA: 3'- aaaACGGUGugGGUCGUGCCGGACGU- -5' |
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21051 | 3' | -57.9 | NC_004745.1 | + | 19366 | 1.06 | 0.000285 |
Target: 5'- cUUUUGCCACACCCAGCACGGCCUGCAa -3' miRNA: 3'- -AAAACGGUGUGGGUCGUGCCGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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