miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21051 5' -50.9 NC_004745.1 + 5685 0.66 0.792916
Target:  5'- uUGCGCGcACuUCAUcaggcaugucAGCCGuGGCgGUCAGu -3'
miRNA:   3'- -ACGCGUuUG-AGUA----------UUGGC-CCG-UAGUC- -5'
21051 5' -50.9 NC_004745.1 + 18323 0.66 0.749082
Target:  5'- cUGCGCGGugUCAgucUGGCUGcGGUcgCAa -3'
miRNA:   3'- -ACGCGUUugAGU---AUUGGC-CCGuaGUc -5'
21051 5' -50.9 NC_004745.1 + 6901 0.66 0.737736
Target:  5'- gUGUGCGgcaucGGCUCcauGCUGGGUAUCGc -3'
miRNA:   3'- -ACGCGU-----UUGAGuauUGGCCCGUAGUc -5'
21051 5' -50.9 NC_004745.1 + 3603 0.67 0.703004
Target:  5'- cGUGCcgAGGCUU---GCCGGGuCAUCGGu -3'
miRNA:   3'- aCGCG--UUUGAGuauUGGCCC-GUAGUC- -5'
21051 5' -50.9 NC_004745.1 + 8425 0.68 0.667538
Target:  5'- aGCGCGuuCUCAagcAGCCGGaUAUCGGc -3'
miRNA:   3'- aCGCGUuuGAGUa--UUGGCCcGUAGUC- -5'
21051 5' -50.9 NC_004745.1 + 8309 0.69 0.582827
Target:  5'- cUGCGCAGgccgggcGCUgGUGcaACCGGcgcacaGCAUCAGg -3'
miRNA:   3'- -ACGCGUU-------UGAgUAU--UGGCC------CGUAGUC- -5'
21051 5' -50.9 NC_004745.1 + 19326 1.09 0.001274
Target:  5'- cUGCGCAAACUCAUAACCGGGCAUCAGc -3'
miRNA:   3'- -ACGCGUUUGAGUAUUGGCCCGUAGUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.