Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21051 | 5' | -50.9 | NC_004745.1 | + | 5685 | 0.66 | 0.792916 |
Target: 5'- uUGCGCGcACuUCAUcaggcaugucAGCCGuGGCgGUCAGu -3' miRNA: 3'- -ACGCGUuUG-AGUA----------UUGGC-CCG-UAGUC- -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 18323 | 0.66 | 0.749082 |
Target: 5'- cUGCGCGGugUCAgucUGGCUGcGGUcgCAa -3' miRNA: 3'- -ACGCGUUugAGU---AUUGGC-CCGuaGUc -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 6901 | 0.66 | 0.737736 |
Target: 5'- gUGUGCGgcaucGGCUCcauGCUGGGUAUCGc -3' miRNA: 3'- -ACGCGU-----UUGAGuauUGGCCCGUAGUc -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 3603 | 0.67 | 0.703004 |
Target: 5'- cGUGCcgAGGCUU---GCCGGGuCAUCGGu -3' miRNA: 3'- aCGCG--UUUGAGuauUGGCCC-GUAGUC- -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 8425 | 0.68 | 0.667538 |
Target: 5'- aGCGCGuuCUCAagcAGCCGGaUAUCGGc -3' miRNA: 3'- aCGCGUuuGAGUa--UUGGCCcGUAGUC- -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 8309 | 0.69 | 0.582827 |
Target: 5'- cUGCGCAGgccgggcGCUgGUGcaACCGGcgcacaGCAUCAGg -3' miRNA: 3'- -ACGCGUU-------UGAgUAU--UGGCC------CGUAGUC- -5' |
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21051 | 5' | -50.9 | NC_004745.1 | + | 19326 | 1.09 | 0.001274 |
Target: 5'- cUGCGCAAACUCAUAACCGGGCAUCAGc -3' miRNA: 3'- -ACGCGUUUGAGUAUUGGCCCGUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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