Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21052 | 3' | -54.4 | NC_004745.1 | + | 11380 | 0.66 | 0.568385 |
Target: 5'- aG-CGGUGAUGAgCCGGUC-GGCaGCAg -3' miRNA: 3'- aCgGCCACUAUU-GGCCGGuUCGaUGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 5690 | 0.66 | 0.556908 |
Target: 5'- cGCUGGUGAUGccgcaggggcGCUGGCgCAc-CUGCAa -3' miRNA: 3'- aCGGCCACUAU----------UGGCCG-GUucGAUGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 18644 | 0.66 | 0.545498 |
Target: 5'- cGCCauGUGAUGAguUCGGUCAGGCU-CAu -3' miRNA: 3'- aCGGc-CACUAUU--GGCCGGUUCGAuGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 10403 | 0.67 | 0.522917 |
Target: 5'- gGCCGGUGucguuguGCUGGCC-GGUUAa- -3' miRNA: 3'- aCGGCCACuau----UGGCCGGuUCGAUgu -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 3001 | 0.67 | 0.51176 |
Target: 5'- cGCC-GUGAUGAauacaCGGCaAGGCUACu -3' miRNA: 3'- aCGGcCACUAUUg----GCCGgUUCGAUGu -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 5099 | 0.67 | 0.51176 |
Target: 5'- gGUCGGUGAUuucuccacACCGGCUAAG--GCGa -3' miRNA: 3'- aCGGCCACUAu-------UGGCCGGUUCgaUGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 21261 | 0.69 | 0.378808 |
Target: 5'- gGCCGGUGugcuuuaucccgaacUGACCGGCggaAAGCUGa- -3' miRNA: 3'- aCGGCCACu--------------AUUGGCCGg--UUCGAUgu -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 20959 | 0.71 | 0.308022 |
Target: 5'- aGCCGGUGccGUGACUGGCUGauaagccugauAGcCUGCAu -3' miRNA: 3'- aCGGCCAC--UAUUGGCCGGU-----------UC-GAUGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 8644 | 0.73 | 0.222445 |
Target: 5'- gUGCCGGU----GCUGGCguGGCUGCGg -3' miRNA: 3'- -ACGGCCAcuauUGGCCGguUCGAUGU- -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 1733 | 0.76 | 0.132482 |
Target: 5'- cGCCGGUGGUGGCCGGU--GGCa--- -3' miRNA: 3'- aCGGCCACUAUUGGCCGguUCGaugu -5' |
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21052 | 3' | -54.4 | NC_004745.1 | + | 20371 | 1.09 | 0.00047 |
Target: 5'- aUGCCGGUGAUAACCGGCCAAGCUACAa -3' miRNA: 3'- -ACGGCCACUAUUGGCCGGUUCGAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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