Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21052 | 5' | -55.3 | NC_004745.1 | + | 20337 | 1.11 | 0.00031 |
Target: 5'- uGGCACCGAUGAUGCCAGUCAGCACCAg -3' miRNA: 3'- -CCGUGGCUACUACGGUCAGUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 20453 | 0.67 | 0.473492 |
Target: 5'- cGCACCGAUGGccGUCAuaaCGGCACUAc -3' miRNA: 3'- cCGUGGCUACUa-CGGUca-GUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 20855 | 0.66 | 0.505834 |
Target: 5'- uGGCACCGGUGGUuaUAuucCGGCgACCAg -3' miRNA: 3'- -CCGUGGCUACUAcgGUca-GUCG-UGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 21805 | 0.68 | 0.422036 |
Target: 5'- cGGCAgcCCGAcc-UGCCcGUCGGCAUCGu -3' miRNA: 3'- -CCGU--GGCUacuACGGuCAGUCGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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