Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21052 | 5' | -55.3 | NC_004745.1 | + | 20855 | 0.66 | 0.505834 |
Target: 5'- uGGCACCGGUGGUuaUAuucCGGCgACCAg -3' miRNA: 3'- -CCGUGGCUACUAcgGUca-GUCG-UGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 6924 | 0.66 | 0.516826 |
Target: 5'- gGGUaucGCCGGuuaucaggugauUGAgauUGCCAuUCAGCGCCGc -3' miRNA: 3'- -CCG---UGGCU------------ACU---ACGGUcAGUCGUGGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 177 | 0.66 | 0.539086 |
Target: 5'- aGGCGgCGAUGuucaGCCGuuGUCAGUGuCCAg -3' miRNA: 3'- -CCGUgGCUACua--CGGU--CAGUCGU-GGU- -5' |
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21052 | 5' | -55.3 | NC_004745.1 | + | 1689 | 0.71 | 0.267623 |
Target: 5'- cGGCcaccACCGgcGGUGCCAGUaccacgcaucCGGCACUg -3' miRNA: 3'- -CCG----UGGCuaCUACGGUCA----------GUCGUGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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