Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21053 | 5' | -54 | NC_004745.1 | + | 8475 | 0.66 | 0.638267 |
Target: 5'- gGCGCUGAcUGauGCCGUC---ACGGUGCUg -3' miRNA: 3'- -CGUGGCU-AC--CGGCAGuauUGCCGUGA- -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 22000 | 0.66 | 0.626694 |
Target: 5'- cGCACCGAgcgUGG-UGUCAUGccaggagccuucACGGCGg- -3' miRNA: 3'- -CGUGGCU---ACCgGCAGUAU------------UGCCGUga -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 30314 | 0.66 | 0.625537 |
Target: 5'- aCACCGuuGUGGaaggcaaCCGUCGcuUAACGGCAg- -3' miRNA: 3'- cGUGGC--UACC-------GGCAGU--AUUGCCGUga -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 28259 | 0.66 | 0.592052 |
Target: 5'- uUACCGcAUGGgCGUCAacGUGGCGCa -3' miRNA: 3'- cGUGGC-UACCgGCAGUauUGCCGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 16754 | 0.67 | 0.580565 |
Target: 5'- uCGCCGGU-GCCGUCuuccACGcGCACa -3' miRNA: 3'- cGUGGCUAcCGGCAGuau-UGC-CGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 754 | 0.67 | 0.557744 |
Target: 5'- gGCG-CGAaGGCUGUCGgcucguugaagGACGGCACc -3' miRNA: 3'- -CGUgGCUaCCGGCAGUa----------UUGCCGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 4652 | 0.67 | 0.557744 |
Target: 5'- cGCGCCGG-GGCU-UCAUuGCGGUAUUu -3' miRNA: 3'- -CGUGGCUaCCGGcAGUAuUGCCGUGA- -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 17943 | 0.67 | 0.535185 |
Target: 5'- gGCGaaaCGGUGGCCGUCG-AAgGGuCAUg -3' miRNA: 3'- -CGUg--GCUACCGGCAGUaUUgCC-GUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 20895 | 0.68 | 0.501982 |
Target: 5'- cCACCGGU-GCCauaCGUGGCGGCAUg -3' miRNA: 3'- cGUGGCUAcCGGca-GUAUUGCCGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 1335 | 0.69 | 0.459192 |
Target: 5'- cGCugCGGcUGGCC-UCAUAgguGGCGCUg -3' miRNA: 3'- -CGugGCU-ACCGGcAGUAUug-CCGUGA- -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 5022 | 0.69 | 0.459192 |
Target: 5'- cGCGgCGAUGGCUGUCAUccauguaguaGAUGG-ACa -3' miRNA: 3'- -CGUgGCUACCGGCAGUA----------UUGCCgUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 11004 | 0.71 | 0.34388 |
Target: 5'- -gGCCGGUGGCCGaccgCGUGACcGUGCa -3' miRNA: 3'- cgUGGCUACCGGCa---GUAUUGcCGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 18367 | 0.73 | 0.250918 |
Target: 5'- uGCACUGAUuaaggugcuGCCGcgCAUGACGGCACc -3' miRNA: 3'- -CGUGGCUAc--------CGGCa-GUAUUGCCGUGa -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 4018 | 0.75 | 0.174636 |
Target: 5'- cCGCCGGUGGCCuugCu--GCGGCGCUg -3' miRNA: 3'- cGUGGCUACCGGca-GuauUGCCGUGA- -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 18438 | 0.76 | 0.151254 |
Target: 5'- aGCAUCGGU-GCCGUCAUGcGCGGCAg- -3' miRNA: 3'- -CGUGGCUAcCGGCAGUAU-UGCCGUga -5' |
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21053 | 5' | -54 | NC_004745.1 | + | 20453 | 1.11 | 0.000472 |
Target: 5'- cGCACCGAUGGCCGUCAUAACGGCACUa -3' miRNA: 3'- -CGUGGCUACCGGCAGUAUUGCCGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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