miRNA: 3'- uaaUACUuuuCGACGC-UACUCUAUGC- -5'
miRNA: 3'- uaAUACUUUUc--GACGCUAC-------UCUAUGC- -5'
miRNA: 3'- uaAUACUUUUCGAc--GCUACU-CUAUGc -5'
miRNA: 3'- uaauACUuUUCGACGCuaCUCUAUGC- -5'
miRNA: 3'- -UAAUACUUUUCGACGCUACUCUAUGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21056 | 5' | -43.7 | NC_004745.1 | + | 29470 | 0.67 | 0.989774 |
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Target: 5'- --aGUGGuu-GCUGCGgAUGGGAUGUGg -3' miRNA: 3'- uaaUACUuuuCGACGC-UACUCUAUGC- -5' |
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| 21056 | 5' | -43.7 | NC_004745.1 | + | 27259 | 0.69 | 0.9649 |
|
Target: 5'- -gUAUGAGGAacuCUGCGAUGccuggguGGAUGCGc -3' miRNA: 3'- uaAUACUUUUc--GACGCUAC-------UCUAUGC- -5' |
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| 21056 | 5' | -43.7 | NC_004745.1 | + | 28686 | 0.69 | 0.961121 |
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Target: 5'- -cUGUGGAAAGCUcacCGGUGGuGAUACu -3' miRNA: 3'- uaAUACUUUUCGAc--GCUACU-CUAUGc -5' |
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| 21056 | 5' | -43.7 | NC_004745.1 | + | 6426 | 0.7 | 0.928704 |
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Target: 5'- ---uUGAuGAGCUGUGgcGGGAUAUGc -3' miRNA: 3'- uaauACUuUUCGACGCuaCUCUAUGC- -5' |
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| 21056 | 5' | -43.7 | NC_004745.1 | + | 27289 | 1.1 | 0.006792 |
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Target: 5'- cAUUAUGAAAAGCUGCGAUGAGAUACGg -3' miRNA: 3'- -UAAUACUUUUCGACGCUACUCUAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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