Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21060 | 3' | -57.1 | NC_004745.1 | + | 8998 | 0.66 | 0.428308 |
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Target: 5'- -gCGAAGcGUCUgcggcaGAgUCAGCAGCGUCGg -3' miRNA: 3'- ugGCUUU-CAGA------CUgGGUCGUCGCGGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 19915 | 0.67 | 0.371447 |
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Target: 5'- aACCGGcucGGUaCUG-CCCAGCAG-GCUGa -3' miRNA: 3'- -UGGCUu--UCA-GACuGGGUCGUCgCGGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 28948 | 0.67 | 0.362483 |
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Target: 5'- gACCGGGucAGUCUGGCCgA-UGGUGCCa -3' miRNA: 3'- -UGGCUU--UCAGACUGGgUcGUCGCGGc -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 9392 | 0.67 | 0.353671 |
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Target: 5'- uGCCGccGGUaUGACCCGGCGGCauCCu -3' miRNA: 3'- -UGGCuuUCAgACUGGGUCGUCGc-GGc -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 2061 | 0.67 | 0.353671 |
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Target: 5'- uGCCGGAGGUCUucucugugcgGACggCCAGUGGCGgCa -3' miRNA: 3'- -UGGCUUUCAGA----------CUG--GGUCGUCGCgGc -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 18429 | 0.68 | 0.336505 |
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Target: 5'- cGCUGGAAcuGcCUGACCUuGUGGCGCUGg -3' miRNA: 3'- -UGGCUUU--CaGACUGGGuCGUCGCGGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 18399 | 0.69 | 0.274 |
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Target: 5'- cACCGAug--CUGACCgAGCAGgagguCGCCGc -3' miRNA: 3'- -UGGCUuucaGACUGGgUCGUC-----GCGGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 4533 | 0.74 | 0.13725 |
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Target: 5'- uGCCGAAAccUCUGACCgCAGCAGCaauCCGa -3' miRNA: 3'- -UGGCUUUc-AGACUGG-GUCGUCGc--GGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 9574 | 0.79 | 0.051741 |
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Target: 5'- uGCCGccGGUCUGcACUCGGCAGCGaCCGg -3' miRNA: 3'- -UGGCuuUCAGAC-UGGGUCGUCGC-GGC- -5' |
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| 21060 | 3' | -57.1 | NC_004745.1 | + | 3945 | 1.07 | 0.000374 |
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Target: 5'- cACCGAAAGUCUGACCCAGCAGCGCCGc -3' miRNA: 3'- -UGGCUUUCAGACUGGGUCGUCGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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