Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21062 | 5' | -54.3 | NC_004745.1 | + | 22254 | 0.67 | 0.507761 |
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Target: 5'- uGGCGGUCGcCgucGCUGCGGGuuauggugACCu -3' miRNA: 3'- -CCGCCAGCcGac-CGACGUCUuua-----UGG- -5' |
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| 21062 | 5' | -54.3 | NC_004745.1 | + | 1308 | 0.68 | 0.49686 |
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Target: 5'- aGGCgaGGUCGGCgUGGCuggcuuccucgcUGCGGcug-GCCu -3' miRNA: 3'- -CCG--CCAGCCG-ACCG------------ACGUCuuuaUGG- -5' |
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| 21062 | 5' | -54.3 | NC_004745.1 | + | 2043 | 0.69 | 0.404188 |
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Target: 5'- uGCGGaCGGCcagUGGCgGCAGAuugucACCa -3' miRNA: 3'- cCGCCaGCCG---ACCGaCGUCUuua--UGG- -5' |
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| 21062 | 5' | -54.3 | NC_004745.1 | + | 8647 | 0.71 | 0.331574 |
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Target: 5'- -cCGGUgcUGGCgUGGCUGCGGGAAaAUCa -3' miRNA: 3'- ccGCCA--GCCG-ACCGACGUCUUUaUGG- -5' |
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| 21062 | 5' | -54.3 | NC_004745.1 | + | 8896 | 0.75 | 0.18809 |
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Target: 5'- gGGCGGgugacCGGCUccGGCgGCGGggGUuCCg -3' miRNA: 3'- -CCGCCa----GCCGA--CCGaCGUCuuUAuGG- -5' |
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| 21062 | 5' | -54.3 | NC_004745.1 | + | 4577 | 1.15 | 0.000222 |
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Target: 5'- uGGCGGUCGGCUGGCUGCAGAAAUACCg -3' miRNA: 3'- -CCGCCAGCCGACCGACGUCUUUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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