Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21064 | 3' | -65.5 | NC_004745.1 | + | 6019 | 0.66 | 0.179631 |
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Target: 5'- cUCUGaaaaGUC-GGGCGGUggACGCCGGUgCAg -3' miRNA: 3'- -GGGCg---CGGuCCCGCCG--UGCGGCCA-GU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 3348 | 0.66 | 0.179631 |
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Target: 5'- gCCGCGCagauguucCAGGuuaauGCGGCaaccauagacACGCgGGUCAa -3' miRNA: 3'- gGGCGCG--------GUCC-----CGCCG----------UGCGgCCAGU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 2988 | 0.66 | 0.157394 |
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Target: 5'- aCCCGCGUCGucaGGCGGCGCuGCUGu--- -3' miRNA: 3'- -GGGCGCGGUc--CCGCCGUG-CGGCcagu -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 20893 | 0.67 | 0.142955 |
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Target: 5'- aCCgGUGCCAuacGuGGCGGCAUGaguccuugcggcgguCUGGUCAa -3' miRNA: 3'- -GGgCGCGGU---C-CCGCCGUGC---------------GGCCAGU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 10589 | 0.67 | 0.141428 |
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Target: 5'- uUCCaGCGUCAGGGUacgGGCGaccgccuccUGCUGGUCu -3' miRNA: 3'- -GGG-CGCGGUCCCG---CCGU---------GCGGCCAGu -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 19161 | 0.68 | 0.126958 |
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Target: 5'- -aCGCGCCAGcGCGuCACgcuGCCGGUUAa -3' miRNA: 3'- ggGCGCGGUCcCGCcGUG---CGGCCAGU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 10263 | 0.68 | 0.110799 |
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Target: 5'- --aGCGCCGGGGUcuGCG-GCUGGUCAa -3' miRNA: 3'- gggCGCGGUCCCGc-CGUgCGGCCAGU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 19453 | 0.71 | 0.071152 |
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Target: 5'- gUUGaUGCCAuucaGGCGGCaACGCCGGUCAc -3' miRNA: 3'- gGGC-GCGGUc---CCGCCG-UGCGGCCAGU- -5' |
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| 21064 | 3' | -65.5 | NC_004745.1 | + | 5910 | 1.09 | 0.000052 |
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Target: 5'- cCCCGCGCCAGGGCGGCACGCCGGUCAg -3' miRNA: 3'- -GGGCGCGGUCCCGCCGUGCGGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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