Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21066 | 3' | -62.5 | NC_004745.1 | + | 19985 | 0.66 | 0.198613 |
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Target: 5'- cGCCGuGCugaugaCUGC-CGCCUCaaccggaaaGCUGGACAa -3' miRNA: 3'- aCGGU-CG------GACGcGCGGAG---------CGACCUGU- -5' |
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| 21066 | 3' | -62.5 | NC_004745.1 | + | 4023 | 0.68 | 0.150969 |
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Target: 5'- cGCCAccGCCgGUG-GCCUUGCUGcGGCGc -3' miRNA: 3'- aCGGU--CGGaCGCgCGGAGCGAC-CUGU- -5' |
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| 21066 | 3' | -62.5 | NC_004745.1 | + | 24172 | 0.68 | 0.133865 |
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Target: 5'- gGCCAGCgaGCagacCGCCgaacuuugagugagUUGCUGGACAu -3' miRNA: 3'- aCGGUCGgaCGc---GCGG--------------AGCGACCUGU- -5' |
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| 21066 | 3' | -62.5 | NC_004745.1 | + | 3825 | 0.69 | 0.131256 |
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Target: 5'- cGCCAcUgaGCaGCGCCUCGCUGaGAUg -3' miRNA: 3'- aCGGUcGgaCG-CGCGGAGCGAC-CUGu -5' |
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| 21066 | 3' | -62.5 | NC_004745.1 | + | 1360 | 0.73 | 0.062115 |
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Target: 5'- aGCCAGCCacGC-CGaCCUCGCcUGGGCGa -3' miRNA: 3'- aCGGUCGGa-CGcGC-GGAGCG-ACCUGU- -5' |
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| 21066 | 3' | -62.5 | NC_004745.1 | + | 7992 | 1.08 | 0.000083 |
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Target: 5'- aUGCCAGCCUGCGCGCCUCGCUGGACAg -3' miRNA: 3'- -ACGGUCGGACGCGCGGAGCGACCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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