miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21068 3' -42.8 NC_004745.1 + 4525 0.67 0.99581
Target:  5'- gGGACuGGCGUUugauAAUGGcg--UUACGGa -3'
miRNA:   3'- -CUUGuCCGUAG----UUACCuuaaAAUGCC- -5'
21068 3' -42.8 NC_004745.1 + 20359 0.69 0.979804
Target:  5'- cAGCAGGCG-CAAUGGGGgcaaucuucacgACGGu -3'
miRNA:   3'- cUUGUCCGUaGUUACCUUaaaa--------UGCC- -5'
21068 3' -42.8 NC_004745.1 + 19743 0.71 0.96228
Target:  5'- -cACAGGC-UCAAUGGcgggg-ACGGg -3'
miRNA:   3'- cuUGUCCGuAGUUACCuuaaaaUGCC- -5'
21068 3' -42.8 NC_004745.1 + 3796 0.77 0.72787
Target:  5'- aGACGGGCGUCAuccUGGccugUUUGCGGc -3'
miRNA:   3'- cUUGUCCGUAGUu--ACCuua-AAAUGCC- -5'
21068 3' -42.8 NC_004745.1 + 9171 1.12 0.007405
Target:  5'- gGAACAGGCAUCAAUGGAAUUUUACGGc -3'
miRNA:   3'- -CUUGUCCGUAGUUACCUUAAAAUGCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.