Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21068 | 3' | -42.8 | NC_004745.1 | + | 4525 | 0.67 | 0.99581 |
Target: 5'- gGGACuGGCGUUugauAAUGGcg--UUACGGa -3' miRNA: 3'- -CUUGuCCGUAG----UUACCuuaaAAUGCC- -5' |
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21068 | 3' | -42.8 | NC_004745.1 | + | 20359 | 0.69 | 0.979804 |
Target: 5'- cAGCAGGCG-CAAUGGGGgcaaucuucacgACGGu -3' miRNA: 3'- cUUGUCCGUaGUUACCUUaaaa--------UGCC- -5' |
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21068 | 3' | -42.8 | NC_004745.1 | + | 19743 | 0.71 | 0.96228 |
Target: 5'- -cACAGGC-UCAAUGGcgggg-ACGGg -3' miRNA: 3'- cuUGUCCGuAGUUACCuuaaaaUGCC- -5' |
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21068 | 3' | -42.8 | NC_004745.1 | + | 3796 | 0.77 | 0.72787 |
Target: 5'- aGACGGGCGUCAuccUGGccugUUUGCGGc -3' miRNA: 3'- cUUGUCCGUAGUu--ACCuua-AAAUGCC- -5' |
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21068 | 3' | -42.8 | NC_004745.1 | + | 9171 | 1.12 | 0.007405 |
Target: 5'- gGAACAGGCAUCAAUGGAAUUUUACGGc -3' miRNA: 3'- -CUUGUCCGUAGUUACCUUAAAAUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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