Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21068 | 5' | -51.1 | NC_004745.1 | + | 11980 | 0.66 | 0.790126 |
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Target: 5'- uGCCGGA---UGCCGGUcagacgaaacugGUGCAUgAGg -3' miRNA: 3'- gCGGCUUuuaGCGGUCA------------CACGUAgUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 6920 | 0.66 | 0.76863 |
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Target: 5'- uGCUGGGuAUCGCCGGU---UAUCAGg -3' miRNA: 3'- gCGGCUUuUAGCGGUCAcacGUAGUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 4125 | 0.67 | 0.723795 |
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Target: 5'- uGCCGAAcugaacggcaucGA-CGCCGGUGaUGUGUCGa -3' miRNA: 3'- gCGGCUU------------UUaGCGGUCAC-ACGUAGUc -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 11283 | 0.68 | 0.653643 |
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Target: 5'- aGCaCGAu-GUCaGCCAGUgGUGCAgUCAGu -3' miRNA: 3'- gCG-GCUuuUAG-CGGUCA-CACGU-AGUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 3601 | 0.69 | 0.594408 |
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Target: 5'- uGCCGAGGcUUGCCGG-GU-CAUCGGu -3' miRNA: 3'- gCGGCUUUuAGCGGUCaCAcGUAGUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 1550 | 0.72 | 0.437055 |
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Target: 5'- aCGCCGAcaccGagGCCGGUG-GCAUCAa -3' miRNA: 3'- -GCGGCUuu--UagCGGUCACaCGUAGUc -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 2061 | 0.72 | 0.410433 |
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Target: 5'- uGCCGGAGGUCuucucugugcggacgGCCAGUG-GCggCAGa -3' miRNA: 3'- gCGGCUUUUAG---------------CGGUCACaCGuaGUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 1734 | 0.75 | 0.282589 |
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Target: 5'- cCGCCGguGGUgGCCGGUG-GCAaauUCAGa -3' miRNA: 3'- -GCGGCuuUUAgCGGUCACaCGU---AGUC- -5' |
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| 21068 | 5' | -51.1 | NC_004745.1 | + | 9205 | 1.09 | 0.001254 |
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Target: 5'- uCGCCGAAAAUCGCCAGUGUGCAUCAGu -3' miRNA: 3'- -GCGGCUUUUAGCGGUCACACGUAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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