miRNA: 3'- aAGU--AGGCCCagUCG-GACCGACUG-GC- -5'
miRNA: 3'- aaGUagGC-CCAGUCGgACCGACUGGc -5'
miRNA: 3'- aAGUaGGcCCAGUCGGaCCGACUGGc -5'
miRNA: 3'- -AAGUA-----GGCCCAGUCGGACCGaCUGgC- -5'
miRNA: 3'- -AAGUAGGCCCAGUCGGACCGACUGGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21071 | 5' | -59.3 | NC_004745.1 | + | 20694 | 0.66 | 0.311163 |
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Target: 5'- gUCAugUUCGGGcaGGCaCUGGCUGACgCGc -3' miRNA: 3'- aAGU--AGGCCCagUCG-GACCGACUG-GC- -5' |
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| 21071 | 5' | -59.3 | NC_004745.1 | + | 1306 | 0.68 | 0.2462 |
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Target: 5'- -cCAggCGaGGUCGGCgUGGCUGGCUu -3' miRNA: 3'- aaGUagGC-CCAGUCGgACCGACUGGc -5' |
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| 21071 | 5' | -59.3 | NC_004745.1 | + | 18233 | 0.71 | 0.141691 |
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Target: 5'- gUUAaCC-GGUCAGCCaGGCUGACCc -3' miRNA: 3'- aAGUaGGcCCAGUCGGaCCGACUGGc -5' |
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| 21071 | 5' | -59.3 | NC_004745.1 | + | 28957 | 0.73 | 0.103215 |
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Target: 5'- aUUCGUuccgaCCGGGUCAGUCUGGCcGAUgGu -3' miRNA: 3'- -AAGUA-----GGCCCAGUCGGACCGaCUGgC- -5' |
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| 21071 | 5' | -59.3 | NC_004745.1 | + | 18171 | 1.07 | 0.000225 |
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Target: 5'- uUUCAUCCGGGUCAGCCUGGCUGACCGg -3' miRNA: 3'- -AAGUAGGCCCAGUCGGACCGACUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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