Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21073 | 5' | -58 | NC_004745.1 | + | 1165 | 0.66 | 0.40971 |
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Target: 5'- gAGC-CGGUGcCGGUACuCGaCCGCCg- -3' miRNA: 3'- gUCGaGCCGCuGUUAUG-GC-GGCGGag -5' |
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| 21073 | 5' | -58 | NC_004745.1 | + | 24118 | 0.66 | 0.381563 |
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Target: 5'- aAGUUCGGCGGuc-UGCuCGCUGgCCUCc -3' miRNA: 3'- gUCGAGCCGCUguuAUG-GCGGC-GGAG- -5' |
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| 21073 | 5' | -58 | NC_004745.1 | + | 11302 | 0.67 | 0.346092 |
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Target: 5'- aGGCUCGgaugagugauucGCGACugcUGCCGaCCGgCUCa -3' miRNA: 3'- gUCGAGC------------CGCUGuu-AUGGC-GGCgGAG- -5' |
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| 21073 | 5' | -58 | NC_004745.1 | + | 19434 | 0.68 | 0.275182 |
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Target: 5'- -cGCcgaGGCGGCGGUAUUGUCGCCg- -3' miRNA: 3'- guCGag-CCGCUGUUAUGGCGGCGGag -5' |
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| 21073 | 5' | -58 | NC_004745.1 | + | 19972 | 0.69 | 0.24107 |
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Target: 5'- cCAGCUCaGCcGCcgccgugcuGAUgACUGCCGCCUCa -3' miRNA: 3'- -GUCGAGcCGcUG---------UUA-UGGCGGCGGAG- -5' |
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| 21073 | 5' | -58 | NC_004745.1 | + | 19374 | 1.09 | 0.000203 |
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Target: 5'- cCAGCUCGGCGACAAUACCGCCGCCUCg -3' miRNA: 3'- -GUCGAGCCGCUGUUAUGGCGGCGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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