Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 21076 | 5' | -43.3 | NC_004746.1 | + | 15327 | 0.74 | 0.826078 |
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Target: 5'- -cAGUUAUuuaaaccuagcagaUGUUGAaaaAGCGAUUGUUCCu -3' miRNA: 3'- aaUCAAUG--------------ACAACU---UCGUUGACAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 25401 | 0.74 | 0.829182 |
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Target: 5'- gUAGUUGCUGaaggUGAAGCGcUUGUUgCCu -3' miRNA: 3'- aAUCAAUGACa---ACUUCGUuGACAA-GG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 19203 | 0.72 | 0.91679 |
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Target: 5'- -aAGUUAUUGUUGuuGCAgaucGCUGgcUUCCa -3' miRNA: 3'- aaUCAAUGACAACuuCGU----UGAC--AAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 13867 | 0.69 | 0.976379 |
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Target: 5'- -aAGUgcUUGUUGgcGUAaaACUGUUCCc -3' miRNA: 3'- aaUCAauGACAACuuCGU--UGACAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 25430 | 1.13 | 0.005684 |
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Target: 5'- uUUAGUUACUGUUGAAGCAACUGUUCCa -3' miRNA: 3'- -AAUCAAUGACAACUUCGUUGACAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 33289 | 0.78 | 0.615476 |
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Target: 5'- -----aGCcGUUGggGUAACUGUUCCg -3' miRNA: 3'- aaucaaUGaCAACuuCGUUGACAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 31178 | 0.69 | 0.98423 |
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Target: 5'- aUAGUcugaGUUGGAGCGACaauaGUUCCa -3' miRNA: 3'- aAUCAaugaCAACUUCGUUGa---CAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 11652 | 0.68 | 0.986347 |
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Target: 5'- ---aUUACUaaagGaAAGCGACUGUUCCa -3' miRNA: 3'- aaucAAUGAcaa-C-UUCGUUGACAAGG- -5' |
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| 21076 | 5' | -43.3 | NC_004746.1 | + | 19908 | 0.68 | 0.989921 |
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Target: 5'- gUAGUUACUGUUuuAGUAAC---UCCa -3' miRNA: 3'- aAUCAAUGACAAcuUCGUUGacaAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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