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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21100 | 3' | -50.8 | NC_004756.1 | + | 1054 | 0.66 | 0.273945 |
Target: 5'- --cAGCCUUGcaagcaAGgGUUCCuucccaguuugguggUCCUGUCAu -3' miRNA: 3'- ucuUCGGAAU------UCgCAAGG---------------AGGACAGU- -5' |
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21100 | 3' | -50.8 | NC_004756.1 | + | 1613 | 0.73 | 0.079302 |
Target: 5'- -uGGGCC--GAGCGUUCCUUCUGUgAa -3' miRNA: 3'- ucUUCGGaaUUCGCAAGGAGGACAgU- -5' |
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21100 | 3' | -50.8 | NC_004756.1 | + | 5403 | 1.1 | 0.000082 |
Target: 5'- cAGAAGCCUUAAGCGUUCCUCCUGUCAa -3' miRNA: 3'- -UCUUCGGAAUUCGCAAGGAGGACAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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