Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21103 | 5' | -54.5 | NC_004761.1 | + | 3780 | 1.08 | 8.1e-05 |
Target: 5'- gUUGUGACCGUUGGGAGGAGGAAGAGGu -3' miRNA: 3'- -AACACUGGCAACCCUCCUCCUUCUCC- -5' |
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21103 | 5' | -54.5 | NC_004761.1 | + | 3893 | 0.71 | 0.094507 |
Target: 5'- ---aGACCGUUuugagGGuGGGGAGGuGGAGGa -3' miRNA: 3'- aacaCUGGCAA-----CC-CUCCUCCuUCUCC- -5' |
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21103 | 5' | -54.5 | NC_004761.1 | + | 3619 | 0.66 | 0.2121 |
Target: 5'- ---aGACCGacgGGGAccuGGAGGAccuggauagGGAGGu -3' miRNA: 3'- aacaCUGGCaa-CCCU---CCUCCU---------UCUCC- -5' |
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21103 | 5' | -54.5 | NC_004761.1 | + | 2694 | 0.75 | 0.037812 |
Target: 5'- -aGUGACCaagaaGAGGAGGAGGAGGg -3' miRNA: 3'- aaCACUGGcaaccCUCCUCCUUCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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