Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21122 | 5' | -56.1 | NC_004775.1 | + | 2671 | 0.66 | 0.553343 |
Target: 5'- -gCAUCaaggGAACCCauccggACCGCUgCGGCa -3' miRNA: 3'- uaGUAGa---CUUGGGca----UGGCGGgGUCG- -5' |
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21122 | 5' | -56.1 | NC_004775.1 | + | 37725 | 0.66 | 0.54234 |
Target: 5'- gGUCAUCUGucuGCCCauaACUGaaCCCGGCu -3' miRNA: 3'- -UAGUAGACu--UGGGca-UGGCg-GGGUCG- -5' |
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21122 | 5' | -56.1 | NC_004775.1 | + | 5543 | 0.67 | 0.499135 |
Target: 5'- cUgGUUUGAACUCGUAUUuCUCCGGCg -3' miRNA: 3'- uAgUAGACUUGGGCAUGGcGGGGUCG- -5' |
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21122 | 5' | -56.1 | NC_004775.1 | + | 834 | 0.7 | 0.353727 |
Target: 5'- cAUCAgccugccacuuUCUGGGCCCGgugGCaUGCgCCAGCu -3' miRNA: 3'- -UAGU-----------AGACUUGGGCa--UG-GCGgGGUCG- -5' |
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21122 | 5' | -56.1 | NC_004775.1 | + | 34206 | 0.71 | 0.296874 |
Target: 5'- --aGUCUGGaccggcuccuGCCCGUGCCaGCCUuuCAGCg -3' miRNA: 3'- uagUAGACU----------UGGGCAUGG-CGGG--GUCG- -5' |
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21122 | 5' | -56.1 | NC_004775.1 | + | 9455 | 1.08 | 0.000546 |
Target: 5'- uAUCAUCUGAACCCGUACCGCCCCAGCc -3' miRNA: 3'- -UAGUAGACUUGGGCAUGGCGGGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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