miRNA: 3'- -GCUGC-ACUua-UGUGaGCuaCAUCGGC- -5'
miRNA: 3'- -GCUGCACUuaUGUGAGCUAC-AUCGgc -5'
miRNA: 3'- -GCUgCACUuAUGu---GAGCUACAUCGGc -5'
miRNA: 3'- gCuGCACUUAUGUG--AGCU----ACauCGGC- -5'
miRNA: 3'- -GCUGCACUUAUGUGAGCUACAUCGGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21126 | 3' | -48.9 | NC_004775.1 | + | 7076 | 0.66 | 0.946725 |
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Target: 5'- aUGACGuUGGuugAUAC-CGcgGUGGCCGu -3' miRNA: 3'- -GCUGC-ACUua-UGUGaGCuaCAUCGGC- -5' |
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| 21126 | 3' | -48.9 | NC_004775.1 | + | 6747 | 0.68 | 0.881783 |
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Target: 5'- gCGACGUGGcaAUGCUUGGUGaUGGCa- -3' miRNA: 3'- -GCUGCACUuaUGUGAGCUAC-AUCGgc -5' |
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| 21126 | 3' | -48.9 | NC_004775.1 | + | 11283 | 0.68 | 0.856499 |
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Target: 5'- gCGAgGUGGuUACcaucUUCGGUGUGGCCc -3' miRNA: 3'- -GCUgCACUuAUGu---GAGCUACAUCGGc -5' |
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| 21126 | 3' | -48.9 | NC_004775.1 | + | 16344 | 0.7 | 0.767592 |
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Target: 5'- uGcCGUGAAUGCACuuUCGGcgcgUGgcGCCGg -3' miRNA: 3'- gCuGCACUUAUGUG--AGCU----ACauCGGC- -5' |
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| 21126 | 3' | -48.9 | NC_004775.1 | + | 17908 | 1.1 | 0.003299 |
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Target: 5'- cCGACGUGAAUACACUCGAUGUAGCCGc -3' miRNA: 3'- -GCUGCACUUAUGUGAGCUACAUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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