Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21127 | 3' | -55.1 | NC_004775.1 | + | 16577 | 0.66 | 0.582684 |
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Target: 5'- aGCCAuuGCgCAgaCACUugGCCGCCAGCc- -3' miRNA: 3'- -CGGUu-CG-GUaaGUGG--CGGCGGUUGuu -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 17311 | 0.66 | 0.575862 |
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Target: 5'- aGCCGAGCgugcaaaagcucaguUAUcuggUCGCCaGCCGCCGAUu- -3' miRNA: 3'- -CGGUUCG---------------GUA----AGUGG-CGGCGGUUGuu -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 7921 | 0.66 | 0.570191 |
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Target: 5'- aGCCAuuGCCAUaacugaaacgcuaUCACCuaCCGCUGGCAGu -3' miRNA: 3'- -CGGUu-CGGUA-------------AGUGGc-GGCGGUUGUU- -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 34333 | 0.67 | 0.526512 |
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Target: 5'- aGUguGGCCAaaggUGCCGCuCGUCAGCAAa -3' miRNA: 3'- -CGguUCGGUaa--GUGGCG-GCGGUUGUU- -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 11733 | 0.68 | 0.441454 |
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Target: 5'- cGCuCAGGCUgaugagGUUCGCCGUCGCaauCGGCAGg -3' miRNA: 3'- -CG-GUUCGG------UAAGUGGCGGCG---GUUGUU- -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 16203 | 0.68 | 0.431367 |
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Target: 5'- cGCCGGGCCAUcCAauCCGCgaGCCAuCAu -3' miRNA: 3'- -CGGUUCGGUAaGU--GGCGg-CGGUuGUu -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 17338 | 0.72 | 0.260943 |
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Target: 5'- uGCC-AGCCAUUCAUCGCgC-CCGGCGu -3' miRNA: 3'- -CGGuUCGGUAAGUGGCG-GcGGUUGUu -5' |
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| 21127 | 3' | -55.1 | NC_004775.1 | + | 21412 | 1.08 | 0.000606 |
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Target: 5'- cGCCAAGCCAUUCACCGCCGCCAACAAu -3' miRNA: 3'- -CGGUUCGGUAAGUGGCGGCGGUUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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