Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21131 | 5' | -54.9 | NC_004775.1 | + | 7902 | 0.66 | 0.569576 |
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Target: 5'- --aCGCUAuCACCUaccGCUGGCAGUg- -3' miRNA: 3'- augGCGGUcGUGGAa--UGACCGUCAau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 8546 | 0.66 | 0.546751 |
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Target: 5'- cUACCaGUCAGCGCCaUAUugaUGGCAGc-- -3' miRNA: 3'- -AUGG-CGGUCGUGGaAUG---ACCGUCaau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 15523 | 0.66 | 0.536573 |
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Target: 5'- cGCCGCCGGgAUCggugcuucagaauCUGGCGGUg- -3' miRNA: 3'- aUGGCGGUCgUGGaau----------GACCGUCAau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 29667 | 0.67 | 0.523107 |
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Target: 5'- uUACCGCCAGCagauacaGCCagGCggaaacGGCGGUc- -3' miRNA: 3'- -AUGGCGGUCG-------UGGaaUGa-----CCGUCAau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 9053 | 0.68 | 0.448597 |
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Target: 5'- cGCCGCUAuGCGCaCgacaACUGGCAGa-- -3' miRNA: 3'- aUGGCGGU-CGUG-Gaa--UGACCGUCaau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 23964 | 0.69 | 0.369193 |
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Target: 5'- aUAaaGCUAGCugUauaugagUUACUGGCAGUUAa -3' miRNA: 3'- -AUggCGGUCGugG-------AAUGACCGUCAAU- -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 29742 | 0.71 | 0.286012 |
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Target: 5'- gACCGCCGuuucCGCCUggcuguaucUGCUGGCGGUa- -3' miRNA: 3'- aUGGCGGUc---GUGGA---------AUGACCGUCAau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 18794 | 0.71 | 0.278485 |
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Target: 5'- aUAUCGCUGGCACaacACUGGCAGg-- -3' miRNA: 3'- -AUGGCGGUCGUGgaaUGACCGUCaau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 26088 | 0.74 | 0.178629 |
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Target: 5'- aACUGCCAGUAaggUGCUGGCGGUa- -3' miRNA: 3'- aUGGCGGUCGUggaAUGACCGUCAau -5' |
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| 21131 | 5' | -54.9 | NC_004775.1 | + | 26152 | 1.05 | 0.000909 |
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Target: 5'- aUACCGCCAGCACCUUACUGGCAGUUAu -3' miRNA: 3'- -AUGGCGGUCGUGGAAUGACCGUCAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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