miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21132 3' -54.6 NC_004775.1 + 5674 0.66 0.654054
Target:  5'- gUGGGCUGCUgACGuuaaagcagacaaaGAgauaGGCGGCGAcaaGCu -3'
miRNA:   3'- -ACUCGACGAgUGC--------------CUa---CCGUUGCU---CG- -5'
21132 3' -54.6 NC_004775.1 + 34998 0.66 0.646078
Target:  5'- gGGGCUGCcgCGCGccUGG-GACGAGUc -3'
miRNA:   3'- aCUCGACGa-GUGCcuACCgUUGCUCG- -5'
21132 3' -54.6 NC_004775.1 + 34464 0.67 0.544207
Target:  5'- aGAGCgaGCUUACuGGGUGGCccACGccaguAGCa -3'
miRNA:   3'- aCUCGa-CGAGUG-CCUACCGu-UGC-----UCG- -5'
21132 3' -54.6 NC_004775.1 + 30655 0.67 0.544207
Target:  5'- cUGAGaUGCUCAggaucugccCGGA--GCGGCGGGCa -3'
miRNA:   3'- -ACUCgACGAGU---------GCCUacCGUUGCUCG- -5'
21132 3' -54.6 NC_004775.1 + 26461 1.12 0.000446
Target:  5'- cUGAGCUGCUCACGGAUGGCAACGAGCu -3'
miRNA:   3'- -ACUCGACGAGUGCCUACCGUUGCUCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.