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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21132 | 3' | -54.6 | NC_004775.1 | + | 5674 | 0.66 | 0.654054 |
Target: 5'- gUGGGCUGCUgACGuuaaagcagacaaaGAgauaGGCGGCGAcaaGCu -3' miRNA: 3'- -ACUCGACGAgUGC--------------CUa---CCGUUGCU---CG- -5' |
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21132 | 3' | -54.6 | NC_004775.1 | + | 34998 | 0.66 | 0.646078 |
Target: 5'- gGGGCUGCcgCGCGccUGG-GACGAGUc -3' miRNA: 3'- aCUCGACGa-GUGCcuACCgUUGCUCG- -5' |
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21132 | 3' | -54.6 | NC_004775.1 | + | 34464 | 0.67 | 0.544207 |
Target: 5'- aGAGCgaGCUUACuGGGUGGCccACGccaguAGCa -3' miRNA: 3'- aCUCGa-CGAGUG-CCUACCGu-UGC-----UCG- -5' |
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21132 | 3' | -54.6 | NC_004775.1 | + | 30655 | 0.67 | 0.544207 |
Target: 5'- cUGAGaUGCUCAggaucugccCGGA--GCGGCGGGCa -3' miRNA: 3'- -ACUCgACGAGU---------GCCUacCGUUGCUCG- -5' |
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21132 | 3' | -54.6 | NC_004775.1 | + | 26461 | 1.12 | 0.000446 |
Target: 5'- cUGAGCUGCUCACGGAUGGCAACGAGCu -3' miRNA: 3'- -ACUCGACGAGUGCCUACCGUUGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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