Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21133 | 3' | -54.2 | NC_004775.1 | + | 37605 | 0.66 | 0.687667 |
Target: 5'- aAUUUCCACCucaGCAgCGUCUGCGu- -3' miRNA: 3'- gUGAAGGUGGucgCGU-GUAGACGUcc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 26291 | 0.66 | 0.6536 |
Target: 5'- uGCUUuuGCCuGCGCAUAUCcgGCGu- -3' miRNA: 3'- gUGAAggUGGuCGCGUGUAGa-CGUcc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 4442 | 0.67 | 0.607929 |
Target: 5'- cCGCUUCCACCGGCauagagcggguGUugAuguUUUGCAGc -3' miRNA: 3'- -GUGAAGGUGGUCG-----------CGugU---AGACGUCc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 14767 | 0.67 | 0.585186 |
Target: 5'- uCACUg-CACCAGUGCGCggCgugGCGGa -3' miRNA: 3'- -GUGAagGUGGUCGCGUGuaGa--CGUCc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 14835 | 0.69 | 0.50737 |
Target: 5'- -cCUUCCGCCAcgccGCGCACuggUGCAGu -3' miRNA: 3'- guGAAGGUGGU----CGCGUGuagACGUCc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 39452 | 0.69 | 0.475343 |
Target: 5'- ---aUCCAUCAGCGCGaaccgaUGCGGGa -3' miRNA: 3'- gugaAGGUGGUCGCGUguag--ACGUCC- -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 18511 | 0.7 | 0.414537 |
Target: 5'- gUACUUCUucgcuACCAGCGCGCAcCUGUg-- -3' miRNA: 3'- -GUGAAGG-----UGGUCGCGUGUaGACGucc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 6489 | 0.73 | 0.277988 |
Target: 5'- cCGCUaCCACCAGCGCugAauacguuuUCUGCGc- -3' miRNA: 3'- -GUGAaGGUGGUCGCGugU--------AGACGUcc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 11363 | 0.76 | 0.195486 |
Target: 5'- gCACUggagaaaUACCAGCGCACAuuUCUGCGGc -3' miRNA: 3'- -GUGAag-----GUGGUCGCGUGU--AGACGUCc -5' |
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21133 | 3' | -54.2 | NC_004775.1 | + | 27826 | 1.09 | 0.00074 |
Target: 5'- uCACUUCCACCAGCGCACAUCUGCAGGc -3' miRNA: 3'- -GUGAAGGUGGUCGCGUGUAGACGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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