miRNA: 3'- -UAGGACACGGa-CGAAACcGUCUaucu -5'
miRNA: 3'- uAGGACACGGACG--AAACCGUcUAUCu -5'
miRNA: 3'- -UAGGACACG-Ga--CGaAACCGUCUaucu -5'
miRNA: 3'- -UAGGACACGGACGaa--ACCGucuaucu -5'
miRNA: 3'- -UAGGACACGGACGAAACCGUCUAUCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21133 | 5' | -52.7 | NC_004775.1 | + | 18990 | 0.67 | 0.604903 |
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Target: 5'- gGUCCUGUGCUgaGCUUUG-CAGAa--- -3' miRNA: 3'- -UAGGACACGGa-CGAAACcGUCUaucu -5' |
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| 21133 | 5' | -52.7 | NC_004775.1 | + | 38289 | 0.68 | 0.593167 |
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Target: 5'- uUCUUGUGCCUGUucUUUGGUA-AUAGu -3' miRNA: 3'- uAGGACACGGACG--AAACCGUcUAUCu -5' |
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| 21133 | 5' | -52.7 | NC_004775.1 | + | 35497 | 0.69 | 0.535258 |
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Target: 5'- cGUCCUGUGCaCgacGC-UUGGCAGAc--- -3' miRNA: 3'- -UAGGACACG-Ga--CGaAACCGUCUaucu -5' |
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| 21133 | 5' | -52.7 | NC_004775.1 | + | 11754 | 0.69 | 0.490402 |
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Target: 5'- aAUCCUGUGCCUGUgccgUGGCc------ -3' miRNA: 3'- -UAGGACACGGACGaa--ACCGucuaucu -5' |
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| 21133 | 5' | -52.7 | NC_004775.1 | + | 27789 | 1.07 | 0.001113 |
|
Target: 5'- gAUCCUGUGCCUGCUUUGGCAGAUAGAu -3' miRNA: 3'- -UAGGACACGGACGAAACCGUCUAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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