Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21134 | 5' | -58.4 | NC_004775.1 | + | 1579 | 0.66 | 0.459552 |
Target: 5'- --uGCGCUACCu--GCGCCGCGGUa- -3' miRNA: 3'- uauUGUGGUGGcacCGCGGUGCCGcg -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 23792 | 0.66 | 0.459552 |
Target: 5'- aAUAACAUCaaGCCGUGGCGUUgcuaugaaaAUGGuUGCu -3' miRNA: 3'- -UAUUGUGG--UGGCACCGCGG---------UGCC-GCG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 13394 | 0.66 | 0.420397 |
Target: 5'- --cGCACCaauaucgacaGCCGUGuGCGCgaugccagUGCGGCGUa -3' miRNA: 3'- uauUGUGG----------UGGCAC-CGCG--------GUGCCGCG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 12446 | 0.66 | 0.420397 |
Target: 5'- gAUGACACUuCCGgaaGGUGCC-CGG-GCu -3' miRNA: 3'- -UAUUGUGGuGGCa--CCGCGGuGCCgCG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 10219 | 0.67 | 0.401591 |
Target: 5'- -cAGCGCUggAUUGUGGCGCC-CaGGCGa -3' miRNA: 3'- uaUUGUGG--UGGCACCGCGGuG-CCGCg -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 26427 | 0.68 | 0.348497 |
Target: 5'- uAUAACGCCAUUGUcGGCG-CugGGuCGUc -3' miRNA: 3'- -UAUUGUGGUGGCA-CCGCgGugCC-GCG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 14454 | 0.69 | 0.30822 |
Target: 5'- --cACGCCGCCuGUGGCGUauuGCGcCGCu -3' miRNA: 3'- uauUGUGGUGG-CACCGCGg--UGCcGCG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 7066 | 0.69 | 0.285085 |
Target: 5'- uUGAUACCGCgGUGGCcguaauggccugaGCCGCGGagaacaaaGCc -3' miRNA: 3'- uAUUGUGGUGgCACCG-------------CGGUGCCg-------CG- -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 30022 | 0.83 | 0.028818 |
Target: 5'- gGUGugG-CGCCGUGGCGCCACGGUGg -3' miRNA: 3'- -UAUugUgGUGGCACCGCGGUGCCGCg -5' |
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21134 | 5' | -58.4 | NC_004775.1 | + | 30093 | 1.08 | 0.000337 |
Target: 5'- aAUAACACCACCGUGGCGCCACGGCGCc -3' miRNA: 3'- -UAUUGUGGUGGCACCGCGGUGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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