Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21135 | 3' | -48.9 | NC_004775.1 | + | 13941 | 0.66 | 0.940519 |
Target: 5'- gGUACCUcGUACCuGUUcaGACAGcGCaGCCg -3' miRNA: 3'- -CAUGGAcUAUGG-CAAa-CUGUU-CG-CGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 38579 | 0.66 | 0.934977 |
Target: 5'- -cGCCgguaGUGCCGgaUGACuggguaauGGUGCCa -3' miRNA: 3'- caUGGac--UAUGGCaaACUGu-------UCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 20144 | 0.66 | 0.929118 |
Target: 5'- -gACaCUGAUuacACCGU--GACuGGUGCCg -3' miRNA: 3'- caUG-GACUA---UGGCAaaCUGuUCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 12754 | 0.66 | 0.927909 |
Target: 5'- -gGCCUGAauCUGUUcaUGACuccagccauagcAGCGCCg -3' miRNA: 3'- caUGGACUauGGCAA--ACUGu-----------UCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 21798 | 0.66 | 0.916448 |
Target: 5'- uUACCUGAuUACCGgUUGGCGcugcuuuuucAcCGCCg -3' miRNA: 3'- cAUGGACU-AUGGCaAACUGU----------UcGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 1745 | 0.67 | 0.9124 |
Target: 5'- -aGCCUGGUACUGgucuucaaagucGGCGAuGCGCUu -3' miRNA: 3'- caUGGACUAUGGCaaa---------CUGUU-CGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 4738 | 0.67 | 0.89508 |
Target: 5'- -aGCCUGAUcCaCGUcgagcUUGuCGAGCGCUu -3' miRNA: 3'- caUGGACUAuG-GCA-----AACuGUUCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 14982 | 0.68 | 0.862356 |
Target: 5'- -aGCCgGAUGCCGguagccagGGCAcAGCaGCCg -3' miRNA: 3'- caUGGaCUAUGGCaaa-----CUGU-UCG-CGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 34042 | 0.68 | 0.834892 |
Target: 5'- -aACCUGAUcGCCGgcgGuCAuaucGCGCCa -3' miRNA: 3'- caUGGACUA-UGGCaaaCuGUu---CGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 5893 | 0.68 | 0.834892 |
Target: 5'- uUGCCU--UGCCGacUUUGAuaaacgcuuuaaCAAGCGCCg -3' miRNA: 3'- cAUGGAcuAUGGC--AAACU------------GUUCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 8764 | 0.68 | 0.834892 |
Target: 5'- -cACCUGAUACU--UUGACAuaucaauGCgGCCa -3' miRNA: 3'- caUGGACUAUGGcaAACUGUu------CG-CGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 36405 | 0.69 | 0.825233 |
Target: 5'- -gGCCUGGccuuUCGcUUUGGCAuccAGCGCCc -3' miRNA: 3'- caUGGACUau--GGC-AAACUGU---UCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 10803 | 0.69 | 0.81534 |
Target: 5'- -cACCUGGUGacuCCagcgUGAC-AGCGCCa -3' miRNA: 3'- caUGGACUAU---GGcaa-ACUGuUCGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 3520 | 0.7 | 0.740636 |
Target: 5'- aUGCCUGAcaucaUGCCGcugGACAGaauGCGCUg -3' miRNA: 3'- cAUGGACU-----AUGGCaaaCUGUU---CGCGG- -5' |
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21135 | 3' | -48.9 | NC_004775.1 | + | 30830 | 1.14 | 0.001321 |
Target: 5'- cGUACCUGAUACCGUUUGACAAGCGCCa -3' miRNA: 3'- -CAUGGACUAUGGCAAACUGUUCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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