miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21135 3' -48.9 NC_004775.1 + 13941 0.66 0.940519
Target:  5'- gGUACCUcGUACCuGUUcaGACAGcGCaGCCg -3'
miRNA:   3'- -CAUGGAcUAUGG-CAAa-CUGUU-CG-CGG- -5'
21135 3' -48.9 NC_004775.1 + 38579 0.66 0.934977
Target:  5'- -cGCCgguaGUGCCGgaUGACuggguaauGGUGCCa -3'
miRNA:   3'- caUGGac--UAUGGCaaACUGu-------UCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 20144 0.66 0.929118
Target:  5'- -gACaCUGAUuacACCGU--GACuGGUGCCg -3'
miRNA:   3'- caUG-GACUA---UGGCAaaCUGuUCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 12754 0.66 0.927909
Target:  5'- -gGCCUGAauCUGUUcaUGACuccagccauagcAGCGCCg -3'
miRNA:   3'- caUGGACUauGGCAA--ACUGu-----------UCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 21798 0.66 0.916448
Target:  5'- uUACCUGAuUACCGgUUGGCGcugcuuuuucAcCGCCg -3'
miRNA:   3'- cAUGGACU-AUGGCaAACUGU----------UcGCGG- -5'
21135 3' -48.9 NC_004775.1 + 1745 0.67 0.9124
Target:  5'- -aGCCUGGUACUGgucuucaaagucGGCGAuGCGCUu -3'
miRNA:   3'- caUGGACUAUGGCaaa---------CUGUU-CGCGG- -5'
21135 3' -48.9 NC_004775.1 + 4738 0.67 0.89508
Target:  5'- -aGCCUGAUcCaCGUcgagcUUGuCGAGCGCUu -3'
miRNA:   3'- caUGGACUAuG-GCA-----AACuGUUCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 14982 0.68 0.862356
Target:  5'- -aGCCgGAUGCCGguagccagGGCAcAGCaGCCg -3'
miRNA:   3'- caUGGaCUAUGGCaaa-----CUGU-UCG-CGG- -5'
21135 3' -48.9 NC_004775.1 + 8764 0.68 0.834892
Target:  5'- -cACCUGAUACU--UUGACAuaucaauGCgGCCa -3'
miRNA:   3'- caUGGACUAUGGcaAACUGUu------CG-CGG- -5'
21135 3' -48.9 NC_004775.1 + 5893 0.68 0.834892
Target:  5'- uUGCCU--UGCCGacUUUGAuaaacgcuuuaaCAAGCGCCg -3'
miRNA:   3'- cAUGGAcuAUGGC--AAACU------------GUUCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 34042 0.68 0.834892
Target:  5'- -aACCUGAUcGCCGgcgGuCAuaucGCGCCa -3'
miRNA:   3'- caUGGACUA-UGGCaaaCuGUu---CGCGG- -5'
21135 3' -48.9 NC_004775.1 + 36405 0.69 0.825233
Target:  5'- -gGCCUGGccuuUCGcUUUGGCAuccAGCGCCc -3'
miRNA:   3'- caUGGACUau--GGC-AAACUGU---UCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 10803 0.69 0.81534
Target:  5'- -cACCUGGUGacuCCagcgUGAC-AGCGCCa -3'
miRNA:   3'- caUGGACUAU---GGcaa-ACUGuUCGCGG- -5'
21135 3' -48.9 NC_004775.1 + 3520 0.7 0.740636
Target:  5'- aUGCCUGAcaucaUGCCGcugGACAGaauGCGCUg -3'
miRNA:   3'- cAUGGACU-----AUGGCaaaCUGUU---CGCGG- -5'
21135 3' -48.9 NC_004775.1 + 30830 1.14 0.001321
Target:  5'- cGUACCUGAUACCGUUUGACAAGCGCCa -3'
miRNA:   3'- -CAUGGACUAUGGCAAACUGUUCGCGG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.