Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21136 | 3' | -45.9 | NC_004775.1 | + | 29045 | 0.66 | 0.988816 |
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Target: 5'- -gAGGCAuuGAAAGUagUCGagGCGUGGg -3' miRNA: 3'- gaUUCGU--CUUUCAaaAGUg-CGCACCg -5' |
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| 21136 | 3' | -45.9 | NC_004775.1 | + | 6350 | 0.67 | 0.971826 |
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Target: 5'- gCUGAGCcuGAAGcUUUCAUGgGccUGGCg -3' miRNA: 3'- -GAUUCGucUUUCaAAAGUGCgC--ACCG- -5' |
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| 21136 | 3' | -45.9 | NC_004775.1 | + | 35231 | 0.68 | 0.96464 |
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Target: 5'- ---uGCGGAuAGUUggcggacuacaUUC-CGCGUGGUg -3' miRNA: 3'- gauuCGUCUuUCAA-----------AAGuGCGCACCG- -5' |
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| 21136 | 3' | -45.9 | NC_004775.1 | + | 28840 | 0.68 | 0.956258 |
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Target: 5'- ---uGCAGgcAGUUUUC-CGCGUaccgcaaacaGGCa -3' miRNA: 3'- gauuCGUCuuUCAAAAGuGCGCA----------CCG- -5' |
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| 21136 | 3' | -45.9 | NC_004775.1 | + | 32420 | 0.69 | 0.929699 |
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Target: 5'- aUGAGCuGGAAGUUccgCACGCuGggugagGGCa -3' miRNA: 3'- gAUUCGuCUUUCAAaa-GUGCG-Ca-----CCG- -5' |
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| 21136 | 3' | -45.9 | NC_004775.1 | + | 31874 | 1.13 | 0.00369 |
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Target: 5'- uCUAAGCAGAAAGUUUUCACGCGUGGCc -3' miRNA: 3'- -GAUUCGUCUUUCAAAAGUGCGCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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